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  • Digitale Medien  (2)
  • fluidized bed adsorption  (1)
  • lysozyme  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Protein separation using metal ion-bound particles in aqueous two-phase system (1999)
    Springer
    Biotechnology techniques 13 (1999), S. 145-148 
    Details
    ISSN: 1573-6784
    Schlagwort(e): protein separation ; aqueous two-phase system ; affinity partitioning ; lysozyme
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008943624956
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  • 2
    Digitale Medien
    Digitale Medien
    Isolation of β-Xylanase from whole broth of Bacillus amyloliquefaciens by adsorption on a matrix in fluidized bed with low degree of expansion (1999)
    Springer
    Bioseparation 8 (1999), S. 273-280 
    Details
    ISSN: 1573-8272
    Schlagwort(e): β-1 ; 4-xylanase ; Bacillus amyloliquefaciens ; cation exchanger ; fluidized bed adsorption ; low bed expansion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract β-1,4-Xylanase, produced extracellularly by Bacillus amyloliquefaciens MIR 32, was isolated directly from the culture broth by adsorption on a cation exchanger, Amberlite IRC-50, in fluidized bed with a low degree of expansion. The enzyme was eluted from the adsorbent by increase in pH, with a recovery of 82.3% and purification of 5.3 fold. About 99.99% of the colony forming units, 82% of the contaminating neutral protease activity, and 100% of the reducing sugars present in the crude feedstock were removed at the end of the purification cycle.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008174513826
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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