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  • 1
    ISSN: 1432-1246
    Keywords: Toluene ; Xylene ; Styrene ; Hippuric acid ; m-Methylhippuric acid ; Phenylglyoxylic acid ; Mandelic acid ; Urinary metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A new high-pressure liquid chromatography (HPLC) method for simultaneous quantitative determination of the urinary metabolites of toluene, m-xylene, and styrene (hippuric acid, m-methylhippuric acid, phenylglyoxylic acid, mandelic acid) is described. The extraction procedure was performed on acidified urines, after addition of 4-hydroxybenzoic acid as internal standard, using a butylchloride/isopropanol mixture and drying 0.5 ml of the organic layer under nitrogen flow. The residue obtained was dissolved in 0.1 ml water/acetonitrile and 5 μl were injected into an HPLC apparatus equipped with a 0.26 × 25 cm HC ODS SIL X column. Absorbance measures were performed at 225 nm throughout the investigation. All metabolites were clearly separated in a short time (12 min) and the amounts of other urinary compounds affecting the analysis were so small that the measurement of low concentrations of the urinary metabolites could be easily performed. Linear calibration curves were obtained from 0.1 to 3 mg/ml and a correlation coefficient greater than 0.99 was found between concentrations of the standards and areas of the peaks. Statistical analysis confirms that this method, which has a high reproducibility, is simple, reliable, and useful for the biologic monitoring of industrial exposure to aromatic compounds.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1590-3478
    Keywords: Myopathy ; toxic agents ; mineral oils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Sommario Viene descritto un caso di miopatia subacuta insorta in un uomo di 47 anni addetto alla manutenzione e pulizia delle caldaie di combustione di una centrale termoelettrica. L'anamnesi lavorativa mette in risalto la rilevanza dell'esposizione all'inalazione delle ceneri, derivate dalla combustione di oli minerali e contenenti vari elementi, metalli e metalloidi, tra cui in particolare vanadio e nichel. La sintomatologia d'esordio, il decorso clinico ed i dati istopatologici muscolari suggeriscono come l'esposizione al tossico abbia una verosimile importanza nella determinazione del quadro.
    Notes: Abstract We report a case of subacute myopathy in a 47 years old man engaged on boiler maintenance at an oil-fired thermoelectric power station. The occupational history highlighted heavy exposure to inhalation of ash derived from mineral oil combustion and containing several elements, metals and metalloids, including vanadium and nickel. The presenting symptoms, clinical course and muscle histopathology suggest that exposure to toxic agents probably played an important part in the causation of the myopathy.
    Type of Medium: Electronic Resource
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