ISSN:
1617-4623
Keywords:
mRNA splicing
;
Intron mutations
;
Ribosomal protein gene
;
Yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing where-as intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333970
Permalink