ISSN:
1617-4623
Schlagwort(e):
mRNA splicing
;
Intron mutations
;
Ribosomal protein gene
;
Yeast
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing where-as intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00333970
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