ISSN:
1420-9071
Keywords:
Hamster oocytes
;
oocytes
;
hamster
;
trilostane
;
oocyte fertilization in vitro
;
oocyte maturation in vitro
;
steroidogenesis
;
pronuclear formation
;
polyspermy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary The effects of the inhibition of steroidogenesis by trilostane on oocyte maturation were examined by studying spontaneous maturation and fertilization in vitro. 10−6 M trilostane had no influence on the meiotic process, whether the oocytes were naked or not. At a concentration of 10−6 M and 10−7 M trilostane, low normal pronuclear formation and high polyspermy were found during in vitro fertilization. However, no retarded male pronuclear development could be detected in the trilostane-treated group. Thus, steroid producing activity within ova is apparently necessary to prevent multiple sperm penetration, but it has no effect on meiosis or the action of the so-called male pronucleus growth factor (MPGF).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01946649
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