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  • 1
    Digitale Medien
    Digitale Medien
    Genetic aspects of the organization of legumin genes in pea (1993)
    Springer
    Plant molecular biology 22 (1993), S. 101-112 
    Details
    ISSN: 1573-5028
    Schlagwort(e): pea ; Pisum sativum ; recombination ; physical & genetic maps ; legumin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to be determined. The relationship between physical and genetic distances in this region is discussed, as are the implications of this for relating physical and genetic maps elsewhere in the pea genome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00038999
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  • 2
    Digitale Medien
    Digitale Medien
    A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis (1990)
    Springer
    Plant molecular biology 15 (1990), S. 707-722 
    Details
    ISSN: 1573-5028
    Schlagwort(e): retrotransposon ; Pisum ; segregation ; PCR ; RFLP ; mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00016121
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  • 3
    Digitale Medien
    Digitale Medien
    Repeated sequences as genetic markers in pooled tissue samples (1993)
    Springer
    Plant molecular biology 22 (1993), S. 153-157 
    Details
    ISSN: 1573-5028
    Schlagwort(e): linkage ; DNA mixtures ; repeated sequences ; pea ; Pisum sativum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We show, using the PDR1 element of pea, that dispersed repeated sequences of moderate copy number can be used simply and efficiently to generate markers linked to a trait of interest. Inspection of hybridization patterns of repeated sequences to DNA mixtures of pooled genotypes is a sensitive way of detecting such markers. The large number of bands in tracks of digests of these mixtures allows the simultaneous sampling of loci at many places in the genome, and the many unlinked loci serve as internal controls. It is also shown that intensity ratios calculated from these band differences can be used to give a rough estimate of linkage distance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00039003
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  • 4
    Digitale Medien
    Digitale Medien
    An RFLP marker for rb in pea (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 362-365 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Pea ; r b ; RFLP ; Vicilin gene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have located an RFLP marker, corresponding to the locus Vc-5, which is linked to the r b locus. We also show that the heterogeneity at the Vc-5 locus is less among r brb lines than among pea genotypes as a whole. The relevance of this RFLP is discussed in relation to the construction of the double recessive rr r brb genotypes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303978
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