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  • Artikel: DFG Deutsche Nationallizenzen  (11)
  • pheromone  (7)
  • Biochemistry and Biotechnology  (4)
  • 1
    Digitale Medien
    Digitale Medien
    Field responses of the western pine beetle1 and one of its predators to host- and beetle-produced compounds (1980)
    Springer
    Journal of chemical ecology 6 (1980), S. 625-641 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Pinus ponderosa ; Dendroctonus brevicomis ; Temnochila chlorodia ; pheromone ; kairomone ; exo-bievicomm ; frontalin ; trans-ver-benol ; verbenone ; terpenes ; Coleoptera ; Scolytidae ; Trogositidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The responses of the western pine beetle (Dendroctonus brev-icomis LeConte) andTemnochila chlorodia (Mannerheim) to candidate attractants—exo- andendo-brevicomm, frontalin,trans-verbenol, ver-benone, and ponderosa pine turpentine and its major monoterpene components—were quantified by counts of beetles on traps baited with the various attractants, singly and in combinations released simultaneously. Combinations ofexo-brevicomin and frontalin plus a monoterpene or turpentine were the most attractive toD. brevicomis. The responses to these attractant combinations were reduced when verbenone plustrans-verbenol were present. All single compounds and binary mixtures, exceptexo-brevicomin plus frontalin, were much less attractive.exo-Brevicomin was most attractive toT. chlorodia, and this response appeared to decrease when verbenone plustrans-verbenol were present.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00987674
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  • 2
    Digitale Medien
    Digitale Medien
    Interruption of response ofDendroctonus brevicomis to its attractive pheromone by components of the pheromone (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 183-196 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Pinus ponderosa ; Dendroctonus brevicomis ; western pine beetle ; attractant ; interruption ; behavior ; pheromone ; Coleoptera ; Scolytidae ; exo-brevicomin ; frontalin ; myrcene ; verbenone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The catch of the western pine beetle,Dendroctonus brevicomis, at an attractive source of racemicexo-brevicomin, racemic frontalin, and myrcene was reduced by surrounding the source with a grid of 48 stations releasing all three compounds together, orexo-brevicomin alone or myrcene alone. Each compound was released at the rate of 2 mg/24 hr/station. The catch at an attractive bolt cut from a tree being colonized byD. brevicomis was not reduced byexo-brevicomin, but was reduced by the combination ofexo-brevicomin, frontalin, and myrcene in one of two tests. When a transect of traps was placed across a 0.81-hectare plot at six of the 48 stations releasing all three compounds, more beetles were caught at outer than at inner traps. More beetles were caught at unbaited traps on trees in a plot when the three compounds were released than when onlyexo-brevicomin or no compounds were released. A few trees were attacked byD. brevicomis in some of the plots. The antiattractant verbenone released from 48 stations at the rate of 4 mg/24 hr/station did not reduce the catch at an attractive tree bolt.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00988646
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  • 3
    Digitale Medien
    Digitale Medien
    Interruption of response ofDendroctonus brevicomis to attractive pheromone by release of pheromone at several rates and spacings (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 85-97 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Coleoptera ; Scolytidae ; bark beetle ; western pine beetle ; pheromone ; interruption ; behavior ; release device
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The number of western pine beetles,Dendroctonus brevicomis, trapped at the center of three 90 × 90-m plots was reduced during a 16-hr period after surrounding an attractive source ofexo-brevicomin, frontalin, and myrcene with 16, 48, or 168 sources of the same attractant. Compounds were released from the center of the plot at 1 mg/16 hr/compound, and from the surrounding sources at 4–280 mg/16 hr/compound. About half of these amounts was released between 1530 hr and 1930 hr, the period of peak beetle flight. No treatment differences were apparent in reduction of catch within the range of release rates and spacings tested. When compounds were released continuously from 168 stations for 17 days, catch at the center of a plot was generally lower than catch before or after this period, but fluctuated daily. More beetles were caught on traps hung on ponderosa pines within a plot and the number of these traps catching beetles was greater when compounds were released from all stations than from only the center station. Beetles caught on traps were attracted into the plots from the surrounding forest, but appeared to be dispersed within the plot when compounds were released from many stations.D. brevicomis attacked at least 91 trees in the plots, of which 25 were killed during two summers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01020353
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  • 4
    Digitale Medien
    Digitale Medien
    Behavior of the western pine beetle during host colonization (1985)
    Springer
    Journal of chemical ecology 11 (1985), S. 1249-1261 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Pinus ponderosa ; Dendroctonus brevicomis ; pheromone ; attractant ; interruptant ; exo-brevicomin ; frontalin ; Coleoptera ; Scolytidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract After living ponderosa pines were baited with either female-infested bolts or synthetic pheromones,Dendroctonus brevicomis were caught on sticky screens throughout trapping periods of 15–46 days; however, large numbers of beetles were trapped during only a small portion (5–10 days) of these trapping periods. The most attractive portions of trees attacked contained 3–6 beetles dm2, in galleries ca. 2 cm long. Catch increased following addition of males to female-infested bolts, supporting the hypothesis that male-produced frontalin is an attractive pheromone of the western pine beetle. Catch at bolts removed from trees under attack was strongly dependent upon levels of boring activity. We found no evidence of interruption of the response to attractants during host colonization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01024113
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  • 5
    Digitale Medien
    Digitale Medien
    Field response ofDendroctonus brevicomis toexo-brevicomin, frontalin, and myrcene released at two proportions and three levels (1985)
    Springer
    Journal of chemical ecology 11 (1985), S. 757-766 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Coleptera ; Scolytidae ; bark beetle ; western pine beetle ; attractant rate ; pheromone ; exo-brevicomin ; frontalin ; myrcene
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Racemicezo-brevicomin, racemic frontalin, and myrcene were released at two proportions (5∶1∶400 and 1∶1∶1), each at three levels (1 ×, 10 ×, and 100 ×) in a ponderosa pine forest in central California. The 5∶1∶400 mix was based on an estimate of the relative amounts released from a ponderosa pine under attack by the western pine beetle,Dendroctonus brevicomis. MoreD. brevicomis were trapped at a source of the three compounds released at 5∶1∶400 than were trapped at a source released at 1∶1∶1, at all three levels, but this difference was statistically significant only at the 1 × and 10 × levels. Sex ratio of trapped beetles and distribution of catch at the source of attractant and 5 m away apparently did not differ between relative release rates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00988303
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  • 6
    Digitale Medien
    Digitale Medien
    Effect of verbenone on response ofDendroctonus brevicomis toexo-Brevicomin, frontalin, and myrcene (1988)
    Springer
    Journal of chemical ecology 14 (1988), S. 113-122 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Coleoptera ; Scolytidae ; bark beetle ; western pine beetle ; pheromone ; attractant ; inhibitor ; exo-brevicomin ; frontalin ; myrcene ; verbenone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract exo-Brevicomin (E), frontalin (F), and myrcene (M) were released at two rates 10-fold apart with verbenone at four rates 10-fold apart, and without verbenone in plots with one trap on a vertical cylinder at the pheromone source and one trap on each of four cylinders 5 m away. Catch of the western pine beetleDendroctonus brevicomis decreased with increasing levels of verbenone at both release rates of EFM, but not all differences in catch were statistically significant. Significantly more beetles were caught at the high rate of EFM than at the low rate, combining all rates of verbenone. The percent of total beetles caught at the center trap tended to decrease with increasing rates of verbenone, but the only statistically significant differences were at the low rate of EFM.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01022535
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  • 7
    Digitale Medien
    Digitale Medien
    TrappingDendroctonus brevicomis Changes in attractant release rate, dispersion of attractant, and silhouette (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 311-321 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Dendroctonus brevicomis ; Temnochila chlorodia ; Coleoptera ; Scolytidae ; Trogositidae ; western pine beetle ; attractant ; pheromone ; trap ; behavior
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A sticky trap with 3 m2 surface area was modified by changes in attractant release rate, vertical dispersion of the attractant, and addition of a tree trunk silhouette to the trap axis. As attractant release rate increased, the number ofDendroctonus brevicomis caught at the source of attractant and at 1.5 and 5.2 m above ground on two vertical silhouettes 4.5 m away increased. In one experiment, more beetles were caught at a dispersed source of attractant than at a point source. Fewer beetles were caught at the lower traps on the two outlying silhouettes when a silhouette was at the source, than when no silhouette was at the source. As attractant release rate increased, the catch of a predator,Temnochila chlorodia, increased at the source.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00988451
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  • 8
    Digitale Medien
    Digitale Medien
    The building of protein structures form α-carbon coordinates (1990)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 7 (1990), S. 366-377 
    Details
    ISSN: 0887-3585
    Schlagwort(e): computer modeling ; protein ; structure ; α-carbons ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A procedure for the construction of complete protein structures from only αcarbon coordinates is described. This involves building the backbone by sequential addition of Pro, Gly, or Ala residues. This main chain structure is then refined using molecular dynamics. Side chains are constructed by sequential addition of atoms with intermediate molecular dynamics refinement. For α lytic protease (a structure that is mostly β sheet) a backbone root mean square deviation (RMSD) of 0.19 Å and an overall RMSD of 1.24 Å from the crystallographic coordinates are attained. For troponin C (67% β-helix), where the coordinates are available only for the α-carbons, a backbone RMSD of 0.41 Å and an overall RMSD of 1.68 Å are attained (fits kindly provided by Dr. Michael James and Natalie Strynadka). For flavodoxin a backbone RMSD of 0.49 Å and an overall RMSD of 1.64 Å were attained.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/prot.340070408
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  • 9
    Digitale Medien
    Digitale Medien
    A simple two-dimensional representation for the common secondary structural elements of polypeptides and proteins (1997)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 27 (1997), S. 227-233 
    Details
    ISSN: 0887-3585
    Schlagwort(e): peptide conformation ; ramachandran plot ; PDB search ; peptide dynamics ; BPTI ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: A simple method is presented for projecting the conformation of extended secondary structure elements of peptides and proteins that extend over four Cαatoms onto a simple two-dimensional surface. A new set of two degrees of freedom is defined, a pseudo-dihedral involving four sequential Cαatoms, as well as the triple scalar product for the vectors describing the orientation of the three intervening peptide groups. The method provides a reduction in dimensionality, from the usual combination of multiple φ,ψ pairs to a single pair, yielding valuable information concerning the structure and dynamics of these important elements. The new two-dimensional surface is explored by reference to 63 selected protein crystal structures together with a comparison of model built peptides representing the common secondary structural elements. Dynamical aspects on this new surface are examined using a molecular dynamics trajectory of Basic Pancreatic Trypsin Inhibitor. © 1997 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Purification of a pyrogen-free human growth hormone antagonist (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 48 (1995), S. 520-528 
    Details
    ISSN: 0006-3592
    Schlagwort(e): human growth hormone ; animal cell culture ; purification ; serum ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Human growth hormone (hGH) is a polypeptide with 191 amino acids and a molecular mass of 22 kilodaltons. With the aid of computer molecular simulation, an hGH analog was created by altering an hGH gene to reflect the change of one amino acid (glycine [G] 120 to arginine [R]) within the third α-helix of the hGH molecule. This hGH analog, named hGHG120R, was found to be an hGH antagonist. It may have important implications in treating human conditions in which hGH levels are abnormally high, as found in type I diabetics. Several hundred milligrams of purified hGHG120R were needed to determine the biological activity of the antagonist in animal models. A multistep downstream process was developed to purify hGHG120R from cultured mouse L cells transfected with the hGHG120R gene. The process consisted of cell clarification, salt precipitation, membrane ultrafiltration, reversed phase high performance liquid chromatography, phase separation, and lyophiliation. This work discusses the rationale for the design of the process and experimental results on the purification of hGHG120R using the process. © 1995 John Wiley & Sons, Inc.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260480515
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