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  • Frost hardening  (2)
  • phosphate  (2)
  • (Neuroepiteloma cell)  (1)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Human FLI-1 localizes to chromosome 11Q24 and has an aberrant transcript in neuroepithelioma
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 1172 (1993), S. 155-158 
    Details
    ISSN: 0167-4781
    Keywords: (Neuroepiteloma cell) ; ETS domain ; Transcription activation ; v-ets
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(93)90283-J
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  • 2
    Electronic Resource
    Electronic Resource
    Frost hardening and photosynthetic performance of Scots pine (Pinus sylvestris L.). II. Seasonal changes in the fluidity of thylakoid membranes (1998)
    Springer
    Planta 204 (1998), S. 201-206 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Electron paramagnetic resonance ; Frost hardening ; Membrane fluidity ; Photoperiod ; Scots pine ; Thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The fluidity of chloroplast thylakoid membranes of frost-tolerant and frost-sensitive needles of␣three- to four-year-old Scots pine (Pinus sylvestris L.) trees, of liposomes produced from the lipids of the thylakoids of these needles, and of liposomes containing varying amounts of light-harvesting complex (LHC) II protein was investigated by means of electron paramagnetic resonance (EPR) measurements using spin-labelled fatty acids as probes. Broadening of the EPR-resonance signals of 16-doxyl stearic acid in chloroplast membranes of frost-sensitive needles and changes in the amplitudes of the peaks were observed upon a decrease in temperature from +30 °C to −10 °C, indicating a drastic loss in rotational mobility. The lipid molecules of the thylakoid membranes of frost-tolerant needles exhibited greater mobility. Moderate frost resistance could be induced in Scots pine needles by short-day treatment (Vogg et al., 1997, Planta, this issue), and growth of the trees under short-day illumination (9 h) resulted in a higher mobility of the chloroplast membrane lipids than did growth under long-day conditions (16 h). The EPR spectrum of thylakoids from frost-tolerant needles at −10 °C was typical of a spin label in highly fluid surroundings. However, an additional peak in the low-field range appeared in the subzero temperature range for the chloroplast membranes of frost-sensitive needles, which represents spin-label molecules in a motionally restricted surrounding. The EPR spectra of thylakoids and of liposomes of thylakoid lipids from frost-hardy needles were identical at +30 °C and −10 °C. The corresponding spectra from frost-sensitive plants revealed an additional peak for the thylakoids, but not for the pure liposomes. Hence, the domains with restricted mobility could be attributed to protein-lipid interactions in the membranes. Broadening of the spectrum and the appearance of an additional peak was observed with liposomes of pure distearoyl phosphatidyl glycerol modified to contain increasing amounts of LHC II. These results are discussed with respect to a loss of chlorophyll and chlorophyll-binding proteins in thylakoids of Scots pine needles under winter conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050247
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Frost hardening and photosynthetic performance of Scots pine (Pinus sylvestris L.) needles. I. Seasonal changes in the photosynthetic apparatus and its function (1998)
    Springer
    Planta 204 (1998), S. 193-200 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Chlorophyll ; Chlorophyll-binding protein ; Frost hardening ; Photosynthesis ; Pinus (frost hardening)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Photosynthetic CO2 uptake, the photochemical efficiency of photosystem II, the contents of chlorophyll and chlorophyll-binding proteins, and the degree of frost hardiness were determined in three-year-old Scots pine (Pinus sylvestris L.) trees growing in the open air but under controlled daylength. The following conditions were compared: 9-h light period (short day), 16-h light period (long day), and natural daylength. Irrespective of induction by short-day photoperiods or by subfreezing temperatures, frost hardening of the trees was accompanied by a long-lasting pronounced decrease in the photosynthetic rates of one-year-old needles. Under moderate winter conditions, trees adapted to a long-day photoperiod, assimilated CO2 with higher rates than the short-day-treated trees. In the absence of strong frost, photochemical efficiency was lower under short-day conditions than under a long-day photoperiod. Under the impact of strong frost, photochemical efficiency was strongly inhibited in both sets of plants. The reduction in photosynthetic performance during winter was accompanied by a pronounced decrease in the content of chlorophyll and of several chlorophyll-binding proteins [light-harvesting complex (LHC)IIb, LHC Ib, and a chlorophyll-binding protein with MW 43 kDa (CP 43)]. This observed seasonal decrease in photosynthetic pigments and in pigment-binding proteins was irrespective of the degree of frost hardiness and was apparantly under the control of the length of the daily photoperiod. Under a constant 9-h daily photoperiod the chlorophyll content of the needles was considerably lower than under long-day conditions. Transfer of the trees from short-day to long-day conditions resulted in a significantly increased chlorophyll content, whereas the chlorophyll content decreased when trees were transferred from a long-day to a short-day photoperiod. The observed changes in photosynthetic pigments and pigment-binding proteins in Scots pine needles are interpreted as a reduction in the number of photosynthetic units induced by shortening of the daily light period during autumn. This results in a reduction in the absorbing capacity during the frost-hardened state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050246
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  • 4
    Electronic Resource
    Electronic Resource
    Reassessment of root competition for P of field-grown maize in pure and mixed cropping (1988)
    Springer
    Plant and soil 106 (1988), S. 299-301 
    Details
    ISSN: 1573-5036
    Keywords: lupin ; maize ; phosphate ; root competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root competition for phosphate (P) in the field was calculated with maize i) as singly grown plants, ii) in pure cropping and iii) in mixed cropping with lupin. The experimentally determined parameters used for this purpose were thein situ root-distance pattern as registered by autoradiography and the P-depletion cylinder around a maize root as determined autoradiographically under radial diffusional flow. The results do not indicate any competition for P between roots of adjacently grown plants in either pure or mixed cropping. On the other hand, the P-depletion cylinders of approximately one third of the roots of an individual maize plant were found to overlap. However, when allowance was made for the concentration gradients within a depletion cylinder, the actual competition for phosphate was less than 1%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02371230
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  • 5
    Electronic Resource
    Electronic Resource
    Development and replenishment of the P-depletion zone around the primary root of maize during the vegetation period (1987)
    Springer
    Plant and soil 101 (1987), S. 247-255 
    Details
    ISSN: 1573-5036
    Keywords: freeze-cutting ; dynamics ; depletion zone ; root longevity ; maize ; P-deficiency ; phosphate ; radioautography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The dynamics of the development and replenishment of P-depletion zones around the primary root of maize (Zea mays L. cv ‘Garbo’) was studied during a vegetation period (80 days) under greenhouse conditions in a loamy sand of low P-availability. A recently described freeze-cutting technique was used to determine radial diffusion of labelled phosphate to the primary root. The development of the depletion zone was biphasic. In the initial phase after two days of growth of the primary root in a soil layer labelled with33P a minimum of isotopically exchangeable P (EP) was observed which had decreased to about 30% of its original amount at the root surface. At that time the corresponding P-concentration in the soil solution was calculated to be as low as 5×10−7 M. The depletion zone had already spread 0.4 mm from the root surface. During the second phase, between the 10th and 20th day of plant growth the concentration of EP at the root surface increased slowly but did not change markedly. However, the depletion zone continued to spread and after the 20th day of growth reached its maximal diameter (1.07 mm from the root surface) but remained completely within the root hair cyclinder; the single root hairs never exceeded 1.14 mm in length. The biphasic growth of the depletion zone was probably caused by proton extrusion of the root tip. Acidification of the soil solution from pH 5.8 to about 3.9 results in an about 3-fold rise of the concentration of desorbed phosphate and might also have activated acidophilic P-translocators of the root during the initial phase. Anion over cation uptake normally prevailing during the later stage of root development might resulted in a rise of the soil pH within the root hair zone. Consequently P-availability, as well as P-uptake capacity declined, but P-uptake by the seminal root still continued until the 20th day. Subsequently, the P-concentration within the depletion zone increased again while simultaneously its extent was reduced until it was almost completely replenished after 60 days indicating a loss of P-uptake capacity of the primary root. Within the root tissue33P was accumulated to about twice the concentration of that in the undepleted soils. This accumulation corresponded to periods of high uptake due to the development of root laterals. In the root cortex a high P-content was observed during the first 30 days of growth. At the onset of the reproductive stage of the plant the P-content of the shoot and especially in the developing seeds rose considerably at the cost of phosphate stored in the root cortex. The accumulation of33P in the root tissue indicated that nutrient gain was mainly achieved during the early stages of plant development and that P was temporarily stored to some extent within the root system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370652
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