ISSN:
1573-5060
Keywords:
Poa pratensis
;
Kentucky bluegrass
;
protoplast
;
plant regeneration
;
suspension culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary A procedure for the regeneration of callus and albino plantlets from protoplasts of Kentucky bluegrass (Poa pratensis L.), cultivar Kimono, is described. Protoplasts were isolated from a suspension culture with shoot-forming ability, which had been initiated from immature inflorescence derived callus. Protoplasts were cultured in modified Kao & Michayluk (KM8P) liquid medium. Plantlets were recovered from protoplast-derived callus growing on hormone-free modified Murashige & Skoog (MS) medium. While the plantlets derived from the original callus cultures were green, those from suspension cultures and suspension culture derived protoplasts were albino.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043380
Permalink