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  • 1
    Electronic Resource
    Electronic Resource
    Apoptosis of cells lacking mitochondrial DNA (1996)
    Springer
    Apoptosis 1 (1996), S. 119-125 
    Details
    ISSN: 1573-675X
    Keywords: Mitochondrial transmembrane potential ; permeability transition ; programmed cell death ; reactive ; oxygen species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mitochondrial genome of animals encodes a few subcomponents of the respiratory chain complexes I, III and IV, whereas nuclear DNA encodes the overwhelming majority, both in quantitative and qualitative terms, of mitochondrial proteins. Complete depletion of mitochondrial DNA (mtDNA) can be achieved by culturing cells in the presence of inhibitors of mtDNA replication or mitochondrial protein synthesis, giving rise to mutant cells (ϱ∘ cells) which carry morphological near-to-intact mitochondria with respiratory defects. Such cells can be used to study the impact of mitochondrial respiration on apoptosis. ϱ∘ cells do not undergo cell death in response to determined stimuli, yet they conserve their potential to undergo full-blown apoptosis in many experimental systems. This indicates that mtDNA and associated functions (in particular mitochondrial respiration) are irrelevant to apoptosis execution. However, the finding that mtDNA-deficient mitochondria can undergo apoptosis does not argue against the involvement of mitochondria in the apoptotic process, since mitochondria from ϱ∘ cells conserve most of their functions including those involved in the execution of the death programme: permeability transition and release of one or several intermembrane proteins causing nuclear apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01321017
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  • 2
    Electronic Resource
    Electronic Resource
    Mitochondrial permeability transition in apoptosis and necrosis (1998)
    Springer
    Cell biology and toxicology 14 (1998), S. 141-145 
    Details
    ISSN: 1573-6822
    Keywords: mitochondrial transmembrane potential ; permeability transition ; programmed cell death ; proteases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Apoptosis has classically been viewed as a process not involving mitochondria, whereas the implication of mitochondrial dysfunction in necrosis has been recognized for several decades. Recently, it has become clear that apoptosis implies a disruption of mitochondrial membrane intregrity that is decisive for the cell death process. Cytofluorometric methods assessing the mitochondrial membrane function and structure can be employed to demonstrate that, at least in most models of apoptosis, mitochondrial changes precede caspase and nuclease activation. Moreover, pharmacological and genetic experiments suggest that the loss of mitochondrial membrane integrity is a critical event of the apoptotic process, beyond or at the point of no return of programmed cell death. Inhibitors of the mitochondrial megachannel (= permeability transition pore) can prevent both the mitochondrial and the post-mitochondrial manifestations of apoptosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007486022411
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Raman and infrared spectroscopic study of the molecular characterization of the biocompatibility of prosthetic biomaterials (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 25 (1994), S. 109-114 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Raman spectra were obtained directly in a non-destructive and non-invasive way from the biomaterials PMMA [poly(methyl methacrylate)], HDPE (high-density polyethylene), α-Al2O3 (alumina) and HA (hydroxyapatite) constituting the components (bone cement, acetabular cup, ball and stem coated with ceramic) of a hip prosthesis. The aim was to give an objective contribution at a molecular level to predict their biocompatibility. In particular, Raman spectra of explanted PMMA bone cements do not show the 1640 cm-1 band due to the stretching mode of C=C unsaturated centres. Moreover, conformational changes of PMMA following the implant appear in the spectra in the range 1100-500 cm-1, where conformational marker bands at 985, 968, 601 and 565 cm-1 are present. The limited number of HDPE explanted acetabular cups did not allow unequivocal Raman results to be obtained by means of band deconvolution in the region between 1500 and 1000 cm-1, regarding the changes in the crystalline, amorphous and interfacial contents of the acetabular cup surfaces following the implant. α-Al2O3 proved to be a ceramic with good biocompatibility as a biomaterial constituting a hip prosthesis. As regards the stem coating, Raman and Fourier transform IR spectra of HA pre-coating and HA coating on metal implants show spectroscopic modifications due to structural and chemical changes with formation of other calcium phosphates. The development of new bioactive coatings containing precursors of bone growth and able to promote osteogenesis is outlined.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250250116
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    Paper (German National Licenses)
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