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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1370-1374 
    ISSN: 1420-9071
    Keywords: Cochliobolus victoriae ; Helminthosporium ; Bipolaris ; Avena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The host-selective phytotoxin victorin, produced by the fungusCochliobolus victoriae, was found to be at least partially peptidic in nature, and did not contain victoxinine. The exact mass of the M-H ion was measured by FABMS as 795.1877. Derivatives of three major acid hydrolysis products were isolated. The structures of the corresponding amino acids were assigned as 2S,3R-3-hydroxyleucine, 5,5-dichloroleucine, and 3-hydroxylysine. A into victorin by the fungus in vivo.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 91 (1995), S. 1129-1136 
    ISSN: 1432-2242
    Keywords: Brassica napus ; somatic hybrids ; protoplast fusion ; Erucic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var. oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R1 generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 94 (1997), S. 1078-1085 
    ISSN: 1432-2242
    Keywords: Key words Black spot ; Intergeneric hybridization ; protoplast fusion ; white mustard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Somatic Hybrids between Sinapis alba and rapid-cycling Brassica oleracea were generated for transferring of resistance to Alternaria brassicae to B. oleracea. A. brassicae causes the significant disease black spot in cruciferous crops. A total of 27 plants were regenerated from protoplast fusion using 0, 5, 10, 20 and 30 krad γ-irradiation of the resistance donor and iodoacetate treatment of B. oleracea. All plants showed intermediate morphology with partially divided leaves and some trichomes on stems and leaves. Flow cytometry and banding patterns of the enzymes leucine amino peptidase (LAP) and phosphoglucose isomerase (PGI) confirmed the hybrid status of the regenerated plants. Some of the plants obtained from cuttings from the somatic hybrids showed a resistance to A. brassicae that was similar to that found in S. alba. The flowers of the somatic hybrids had reduced anthers with little pollen production.
    Type of Medium: Electronic Resource
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