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  • 1
    Electronic Resource
    Electronic Resource
    Cytoplasmic DNAs and nuclear rDNA restriction fragment length polymorphisms in commercial witloof chicories (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 505-509 
    Details
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; Mitochondrial DNA ; rDNA ; RFLP ; Witloof chicory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphisms of cytoplasmic DNAs and nuclear rDNA were analyzed in several Cichorium intybus genotypes, comprising four white inbred lines, eight red witloof experimental lines, and a number of F1 hybrids derived from two white parents. Chloroplast and mitochondrial restriction patterns led to the distinction between two different cytoplasms, called I and II. Southern hybridization using a nuclear rDNA probe revealed that all the lines possessed two types of rDNA repeat units. The shortest unit was 10 kb and was common to all lines. The largest rDNA repeat unit was 10.5 kb in lines I and 10.4 kb in lines II. In addition, a sequence heterogeneity between the 10.5 and 10.4-kb rDNA repeat units was revealed by Sac I digestion. A 10-kb rDNA unit was successively cloned, mapped, and used as a probe to check the genetic purity of F1 hybrid seeds between line I and II white parents. We found a 30% average percentage of impurities, originating both from selfing and full-sib crossing, in different open-pollinated hybrid samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222980
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 602-608 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; RFLP ; rDNA ; Tobacco mosaic virus ; Introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We studied rDNA restriction fragment length polymorphism between two tomato lines used for F1 hybrid seed production: line A, containing the Tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species Lycopersicon hirsutum, and line B, a tobacco mosaic virus sensitive line. Hybridization patterns led to distinct rDNA maps with two size classes, 10.4 and 10.7 kb, in line A and a single, 8.9-kb class in line B. Size differences were located in the intergenie sequence (IGS). A highly specific 54-bp TaqI fragment was cloned from the line A IGS and used in dot blot experiments to probe total DNA from line A, line B, and their F1 hybrid. It proved capable of discriminating B from A and the hybrid. This probe could thus serve to screen inbreds in commercial seed lots where line A is used as male. This fragment showed 80–90% sequence homology with the 53-bp subrepeats previously characterized in a region of the tomato IGS close to the 25S rRNA gene. Preliminary comparison of rDNA in line A and several wild related species indicated that the L. hirsutum H2 genotype was the closest to line A. rDNA variations between line A and this wild genotype could be explained by recombination during the introgression process involving numerous backcrosses or by an important intraspecific polymorphism. Our results strongly suggest that Tm-1 and the rDNA were introgressed together into tomato from L. hirsutum through linkage drag.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224218
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 546-552 
    Details
    ISSN: 1432-2242
    Keywords: Brassica napus ; Brassica hirta ; Somatic hybridization ; mtDNA ; ctDNA ; rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybridization between Brassica napus and B. hirta (or Sinapis alba) is described. No cybrid plant with B. napus nucleus exhibiting cytoplasmic male sterility was recovered. Somatic hybrids were identified morphologically and, for some of them, by cytological observations. They were also characterised by Southern hybridization of nuclear rDNA. Chloroplast and mitochondrial DNA restriction analysis showed that 2 plants out of 14 have B. hirta ctDNA, one the B. napus mtDNA and the other a hybrid. Nine possess B. napus ctDNA with a hybrid mtDNA. For six of them, mtDNA patterns present novel bands, suggesting intergenomic recombination during fusion. These hybrids will be included in the breeding program.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289119
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    Paper (German National Licenses)
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