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  • 1
    Electronic Resource
    Electronic Resource
    Resource quality and spatial variation in sex ratios of a free-living solitary sawfly, Dineura virididorsata (Hym., Tenthredinidae) (1993)
    Springer
    Oecologia 96 (1993), S. 420-425 
    Details
    ISSN: 1432-1939
    Keywords: Biased sex ratios ; Dineura virididorsata ; Sex-related survival ; Local mate competition ; Foliage quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sex ratio in final-instar larvae of a birch-feeding, free-living solitary sawfly, Dineura virididorsata, was investigated in Finnish Lapland. The prepupal proportion of females, pooled over ten sites, was 56%, and at four individual sites the sex ratio was significantly female-biased. Larval survival from egg to prepupae did not differ between the sexes. This suggests a femalebiased primary sex ratio in the field. The sex ratio varied among the sites but not among host trees within sites. Contrary to previous results with hymenopterans, we did not find that differences in the sex ratio depended on forage quality: site-specific or tree-specific sex ratios did not correlate with the average prepupal weight. A literature search indicated that female-biased sex ratios are also common in other free-living sawflies. We are unable to explain sex ratios of Dineura virididorsata or other free-living sawflies with existing general models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317514
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  • 2
    Electronic Resource
    Electronic Resource
    Reproductive behaviour of mated and virgin females of a solitary sawflyDineura virididorsata (1994)
    Springer
    Entomologia experimentalis et applicata 70 (1994), S. 83-90 
    Details
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Symphyta ; Tenthredinidae ; Dineura virididorsata ; sawfly ; sex ratio ; oviposition behaviour ; mating behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex ratios of the arrhenotokous sawflyDineura virididorsata Retz. (Hymenoptera: Tenthredinidae) are variable among populations in the field, but are biased in favour of females. We have concluded elsewhere that the sex ratios of this species cannot be satisfactorily explained by any general sex ratio model that is available. Here we present data on the different behavioural traits that are relevant to interpretation of the evolution of female-biased sex ratios under local mate competition. We report on oviposition behaviour, mating behaviour and the patterns of movement of ovipositing virgin and mated females. Our results show that adult emergence is synchronous and females will mate daily with different males (in the laboratory, at least). This would decrease the relatedness of offspring should it also take place in the field. In addition, ovipositing females (mated and virgin) leave their site of release in a relatively short time and deposit few eggs relative to their capabilities, so siblings are unlikely to be clumped. The time that virgins spend in deposition of an egg is not different from that spent by mated females, so there is no differential ‘investment’ in this regard. These behavioural observations agree with our previous conclusion that the preconditions of current sex ratio theories are not met byD. virididorsata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380634
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  • 3
    Electronic Resource
    Electronic Resource
    Comparison of two juvenile hormone radioimmunoassays (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 23 (1993), S. 147-152 
    Details
    ISSN: 0739-4462
    Keywords: honey bee ; Apis mellifera ; juvenile hormone ; radioimmunoassay ; hemolymph ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Juvenile hormone from the hemolymph of adult worker honey bees of known age and behavioral status was extracted and analyzed by two different radioimmunoassays in two independent laboratoies. The assays are different in hapten attachment, radiolabeled tracer, and the method by which bound and unbound hormone are separated. Despite these differences in the methods, hormone determinations were in excellent agreement at lower levels (0-50 ng/ml) but diverged as the hormone concentrations increased (〉 50 ng/ml). The relative changes are in good agreement, with a correlation coefficient of 0.97. © 1993 Wiley-Liss, Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940230306
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  • 4
    Electronic Resource
    Electronic Resource
    Juvenile hormone regulation of hemolymph juvenile hormone binding protein in the black strain of the tobacco hornworm, Manduca sexta (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 165-176 
    Details
    ISSN: 0739-4462
    Keywords: Lepidoptera ; radioimmunoassay ; enzyme immunoassay ; equilibrium dialysis ; monoclonal antibody ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Numerous studies have demonstrated regulation of specific lepidopteran proteins by pharmacological doses of insect juvenile hormone (JH). In this study, topical application of a 1 pg dose of JH I to fourth stadium larvae of the black (bl) mutant strain of the tobacco hornworm, Manduca sexta, induced a 50% increase in the titer of hemolymph juvenile hormone binding protein (hJHBP). Radioimmunoassay confirmed that JH titers were lower in bl larvae than in wild-type larvae at the time of JH treatment. Enzyme immunoassay analysis of hJHBP titers demonstrated that regulation by JH I was dose-dependent at doses up to 10 pg and that the response was saturated above 100 pg. Western blotting and equilibrium dialysis confirmed these results and demonstrated that hJHBP from bl larvae had the same molecular mass and displayed the same affinity for JH I as hJHBP isolated from wild-type larvae. Time course studies showed that regulation was complex: 1 2 h after JH I treatment, hJHBP titers were twofold lower in treated than in control bl larvae, while 44 h after treatment they were twofold higher. JH I regulation of hJHBP titers in bl larvae was independent of changes in total hemolymph protein. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940300207
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  • 5
    Electronic Resource
    Electronic Resource
    Recent advances in radioimmunoassay technology for the juvenile hormones (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 30 (1995), S. 295-306 
    Details
    ISSN: 0739-4462
    Keywords: radioimmunoassay ; JH I ; JH II ; JH III ; hemolymph ; insect hormone ; Manducasexta sexta ; Hyalophora cecropia ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Recent refinements in juvenile hormone radioimmunoassay technology now make this method significantly more sensitive and easier to use. Rabbit poly-clonal antisera against (10R) JH III and racemic JH II have been developed to determine hemolymph hormone titers in the low picogram range. The antisera display minimal cross-reactivity with JH metabolites, JH analogs, and hemolymph lipids. One antiserum recognizes racemic JH I, II, and (10R) III almost equivalently, exhibiting 50% displacement between 100 and 130 pg per tube. Another antiserum is JH II-specific and exhibits 50% displacement at 35 pg per tube. Assay sensitivity has been enhanced by using (10R,11S) [methyl-3H]-JH II of very high specific activity (〉 80 Ci/mmol) generated with Hyalophora cecropia accessory gland S-adenosylmethionine transferase and S-[methyl-3H]-adenosyl-L-methionine. Preparation of biological samples has been simplified with overall recoveries of JH from hemolymph ranging between 60 and 75%. © 1995 Wiley-Liss, Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/arch.940300215
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