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  • 1
    Electronic Resource
    Electronic Resource
    Concentrations of fumonisin B1 in feeds associated with animal health problems (1991)
    Springer
    Mycopathologia 114 (1991), S. 129-135 
    Details
    ISSN: 1573-0832
    Keywords: Fumonisin B1 fumonisin B2 ; equine leukoencephalomalacia ; swine pulmonary edema ; animal feeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ninety-eight samples of feeds associated with 44 cases of equine leukoencephalomalacia (ELEM) and 83 samples of feed associated with 42 cases of a porcine pulmonary edema syndrome (PPE) were analyzed for fumonisin B1 (FB1). For comparison purposes, 51 feed samples not associated with PPE or ELEM were also analyzed. Feed associated with ELEM contained FB1 ranging from less than 1 μg/g to 126 μg/g with 75% of the cases having at least 1 sample above 10 μg/g. Feeds associated with PPE ranged from less than 1 μg/g to 330 μg/g with 71% of the cases having at least 1 sample greater than 10 μg/g. Quantitation was by high performance liquid chromatography (HPLC)/fluorescence using the fluorescamine derivative with confirmation by thin layer chromatography (TLC) and/or gas chromatography/mass spectroscopy (GC/MS).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437200
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transport by reconstituted lactose carrier from parental and mutant strains ofEscherichia coli (1984)
    Springer
    The journal of membrane biology 79 (1984), S. 185-193 
    Details
    ISSN: 1432-1424
    Keywords: reconstitution ; lactose transport ; membrane potential ; pH gradient ; proteolytic enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The lactose transport carrier from parental (X71/F'W3747) and mutant cells (54/F'5441) was reconstituted into proteoliposomes. Transport by the counterflow assay showed slightly greater activity in proteoliposomes prepared from extracts of the mutant membranes compared with that for the parental cell. The mutant carrier showed a threefold lowerK m but similarV max compared to the parent. On the other hand proteoliposomes from the mutant showed a defect in protonmotive force-driven accumulation, compared with the parent. With a pH gradient (inside alkaline) plus a membrane potential (inside negative) the parental proteoliposomes accumulated lactose 25-fold over the medium concentration while the mutant proteoliposomes accumulated sixfold. In a series of experiments proteoliposomes were exposed to proteolytic enzymes. Chrymotrypsin treatment resulted in 30% inhibition of counterflow activity for the reconstituted carrier from both parent and mutant. Papain produced 84% inhibition of transport by the reconstituted parental carrier but only 41% of that of the mutant. Trypsin and carboxypeptidase Y treatment had no effect on counterflow activity of either parent or mutant. Exposure of purified lactose carrier in proteoliposomes to carboxypeptidase Y resulted in the release of alanine and valine, the two C-terminal amino acids predicted from the DNA sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872122
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of different phospholipids on the reconstitution of two functions of the lactose carrier ofEscherichia coli (1985)
    Springer
    The journal of membrane biology 84 (1985), S. 259-267 
    Details
    ISSN: 1432-1424
    Keywords: proteoliposomes ; counterflow ; lactose carrier ; phospholipid requirement ; Escherichia coli ; reconstitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The lactose carrier was extracted from membranes ofEscherichia coli and transport activity reconstituted in proteoliposomes containing different phospholipids. Two different assays f for carrier activity were utilized: counterflow and membrane potential-driven uptake. Proteoliposomes composed ofE. coli lipid or of 50% phosphatidylethanolamine−50% phosphatidylcholine showed very high transport activity with both assays. On the other hand, proteoliposomes containing asolectin, phosphatilcholine or 25% cholesterol/75% phosphatidylcholine showed good counterflow activity but poor membrane potentialdriven uptake. The discrepancy between the two types of transport activity in the latter group of three lipids is not due to leakiness to protons, size of proteoliposomes, or carrier protein content per proteoliposome. Apparently one function of the carrier molecule shows a broad tolerance for various phospholipids, while a second facet of the membrane protein activity requires very restricted lipid enviroment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871389
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Experimental reproduction of ELEM (1992)
    Springer
    Mycopathologia 117 (1992), S. 115-120 
    Details
    ISSN: 1573-0832
    Keywords: Fumonisins ; equine leukoencephalomalacia ; mycotoxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An experiment to gain insight into the minimum toxic dose of fumonisins was conducted by feeding ponies rations with known fumonisin concentrations. Naturally contaminated corn screenings (CS) were blended with pellets, corn, and molasses to formulate individual daily diets. One group of 4 ponies was fed a ration with fumonisin B1 (FB1) varying from 〈1 ppm to 22 ppm. A second group of 5 ponies was fed a ration at varying rates containing 8 ppm FB1 for 180 days. A panel of clinical chemistry parameters was evaluated twice weekly for both groups. One pony in the first group died of equine leukoencephalomalacia (ELEM) after 225 days of which the final 55 days' diet contained 22 ppm FB1. Approximately 9 days prior to death, this animal experienced elevated liver chemistry values. All 5 ponies in the second group experienced mild, transient, clincal signs; were euthanized at 180 days; and had mild, histopathological brain lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00497287
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    Paper (German National Licenses)
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