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  • satellite DNA  (4)
  • evolutionary algorithms  (2)
  • 25.70.Lm  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 9 (1998), S. 289-294 
    ISSN: 1572-8145
    Keywords: Manufacturing process chain ; modelling ; optimization ; neural networks ; evolutionary algorithms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Today's manufacturing methods are caught between the growing need for quality, high process safety, minimal manufacturing costs, and short manufacturing times. In order to meet these demands, process setting parameters have to be chosen in the best possible way, according to demand on quality. For such optimization it is necessary to represent the processes in a model. Due to the enormous complexity of many processes and the high number of influencing parameters, however, conventional approaches to modelling and optimization are no longer sufficient. In this article it is shown how, by means of applying neural networks for process modelling, even these highly complex interdependencies can be learned. That way both process and quality parameters can be assessed before or during processing. By connecting them with corresponding cost models, it is possible to optimize processes with the help of evolutionary algorithms. Using examples of different manufacturing processes, the possi bilities for process modelling and optimization with neural networks and evolutionary algorithms are demonstrated.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of intelligent manufacturing 9 (1998), S. 331-338 
    ISSN: 1572-8145
    Keywords: Simulation ; modelling ; machine learning ; evolutionary algorithms ; artificial neural network
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The use of simulation technology as a tool for planning and control is of increasing significance in most fields of production. The main part of the expenditure concerning simulation analyses is the modelling of the considered production. Despite the use of modern building-block-oriented modelling technology, this modelling can often not be done by the user, but only by external experts. Against this backdrop, an adaptive simulation system is being developed by the Institute for Industrial Manufacturing and Management (IFF) at the University of Stuttgart. It independently adapts to real production processes, i.e. it learns about the interdependencies of production processes, and, in this way, supports the user in constructing and maintaining the model. In terms of information technology, the research in the field of artificial intelligence, especially in the subdomain of machine learning, is the basis for the realization of such adaptive systems.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5028
    Keywords: Beta procumbens ; Beta vulgaris ; in situ hybridization ; repetitive DNA ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Members of three prominent DNA families of Beta procumbens have been isolated as Sau3A repeats. Two families consisting of repeats of about 158 bp and 312 bp are organized as satellite DNAs (Sau3A satellites I and II), whereas the third family with a repeat length of 202 bp is interspersed throughout the genome. Multi-colour fluorescence in situ hybridization was used for physical mapping of the DNA families, and has shown that these tandemly organized families occur in large heterochromatic and DAPI positive blocks. The Sau3A satellite I hybridized exclusively around or near the centromeres of 10, 11 or 12 chromosomes. The Sau3A satellite family I showed high intraspecific variability and high-resolution physical mapping was performed on pachytene chromosomes using differentially labelled repeats. The physical order of satellite subfamily arrays along a chromosome was visualized and provided evidence that large arrays of plant satellite repeats are not contiguous and consist of distinct subfamily domains. Re-hybridization of a heterologous rRNA probe to mitotic metaphase chromosomes revealed that the 18S-5.8S-25S rRNA genes are located at subterminal position on one chromosome pair missing repeat clusters of the Sau3A satellite family I. It is known that arrays of Sau3A satellite I repeats are tightly linked to a nematode (Heterodera schachtii) resistance gene and our results show that the gene might be located close to the centromere. Large arrays of the Sau3A satellite II were found in centromeric regions of 16 chromosomes and, in addition, a considerable interspersion of repeats over all chromosomes was observed. The family of interspersed 202 bp repeats is uniformly distributed over all chromosomes and largely excluded from the rRNA gene cluster but shows local amplification in some regions. Southern hybridization has shown that all three families are specific for genomes of the section Procumbentes of the genus Beta.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5028
    Keywords: Arabidopsis arenosa ; Cruciferae ; genus Arabidopsis ; in situ hybridization ; molecular systematics ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analysed a family of highly repetitive DNA from Arabidopsis arenosa (L.) Lawalrée [syn. Cardaminopsis arenosa (L.) Hayck] composed of AT-rich tandem repeats of 166–179 bp in head to tail organization. Sequence comparison between several repeat units revealed a high level of divergence of 4.5% to 25%. The sequence family shows more than 58% homology to satellite sequences described in Arabidopsis thallana (L.) Heynh. but no homology to other satellite repeats in the Cruciferae. Within the genus Arabidopsis the satellite sequence was found to be present in A. thaliana and Arabidopsis suecica (Fries) Norrlin, but not in Arabidopsis griffithiana (Boiss.) N. Busch and Arabidopsis pumila (Stephan) N. Busch. In situ hybridization to metaphase chromosomes of A. arenosa (2n=4x=32) showed the sequence to be localized at the centromeres of all 32 chromosomes with substantial hybridization along the chromosome arms. Using Southern hybridization and in situ hybridization, we give evidence that A. suecica is a hybrid of A. thaliana and A. arenosa. A considerable reorganization of the A. thaliana satellite sequence pAL1 occurred in the hybrid genome while no molecular change of the A. arenosa repeat was observed in the hybrid. Analysis of related repeats enabled differentiation between closely related genomes and are useful for the investigation of hybrid genomes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5028
    Keywords: Cicer arietinum L. ; repetitive DNA ; retrotransposable element ; satellite DNA ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three major repetitive DNA sequences were isolated from a genomic library of chickpea (Cicer arietinum L.) and characterized with respect to their genomic organization and chromosomal localization. All repetitive elements are genus-specific and mostly located in the AT-rich pericentric heterochromatin. Two families are organized as satellite DNAs with repeat lengths of 162–168 bp (CaSat1) and 100 bp (CaSat2). CaSat1 is mainly located adjacent to the 18S rDNA clusters on chromosomes A and B, whereas CaSat2 is a major component of the pericentric heterochromatin on all chromosomes. The high abundance of these sequences in closely related species of the genus Cicer as well as their variation in structure and copy number among the annual species provide useful tools for taxonomic studies. The retrotransposon-like sequences of the third family (CaRep) display a more complex organization and are represented by two independent sets of clones (CaRep1 and CaRep2) with homology to different regions of Ty3-gypsy-like retrotransposons. They are distributed over the pericentric heterochromatin block on all chromosomes with extensions into euchromatic regions. Conserved structures within different crossability groups of related Cicer species suggest independent amplification or transposition events during the evolution of the annual species of the genus.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1434-601X
    Keywords: 25.70.Lm ; Mn
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Correlations between two intermediate-mass fragments resulting from 840 MeV32S projectiles interacting with197Au were measured. The angular correlations and absolute two-fragment cross sections agree with predictions of a semi-microscopic model for direct dynamic projectile break-up. This suggests a predominantly direct mechanism for the production of intermediate-mass fragment pairs. It is argued that the intensity of such a channel which, compared to decays yieldingα particles, is much larger than observed for the sequential decay of corresponding primary excited nuclei, supports the direct-break-up interpretation. The model mechanism yields also strongly damped projectile fragments of intermediate mass.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-6849
    Keywords: Beta species ; marker-assisted selection ; molecular cytogenetics ; nematode resistance ; satellite DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromatin originating from wild beets of the genus Beta, section Procumbentes, has been investigated in nematode-resistant hybrid-derived lines of sugar beet (Beta vulgaris L.) by in situ hybridization using satellite, telomeric and ribosomal DNA repeats, a yeast artificial chromosome (YAC) and total genomic DNA as probes. The alien chromosome was detected in three monosomic addition lines(2n=18+1) by genomic in situ hybridization. Fluorescence in situ hybridization with a genome-specific satellite repeat and YAC DNA enabled the visualization of Procumbentes chromosomes, and in double-target hybridization it was shown that they do not carry 18S–5.8S–25S rRNA and 5S rRNA genes. The wild beet-specific satellite repeat and the telomere sequence from Arabidopsis thaliana were used to perform a structural analysis of the wild beet chromosome fragments of two resistant fragment addition lines. It was shown that one physical end of the chromosome fragments consists of telomeric repeats. Comparison of fragment sizes indicated that the small chromosome fragments harbouring the resistance gene most likely resulted from the loss of one wild beet chromosome arm and an internal deletion of the remaining arm.
    Type of Medium: Electronic Resource
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