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  • 1
    Electronic Resource
    Electronic Resource
    Benzyl-N-acetyl-α-d-galactosaminide inhibits the sialylation and the secretion of mucins by a mucin secreting HT-29 cell subpopulation (1996)
    Springer
    Glycoconjugate journal 13 (1996), S. 717-726 
    Details
    ISSN: 1573-4986
    Keywords: HT-29 cells ; mucins ; aryl-glycosides ; O-glycosylation ; sialyltransferases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract We have analysed the mucins synthesized by the HT-29 MTX cell subpopulation, derived from the HT-29 human colon carcinoma cells through a selective pressure with methotrexate (Lesuffleuret al., 1990,Cancer Res 50: 6334–43), in the presence of benzyl-N-acetyl-α-galactosaminide (GalNAcα-O-benzyl), which is a potential competitive inhibitor of the β1,3-galactosyltransferase that synthesizes the T-antigen. The main observation was a 13-fold decrease in the sialic acid content of mucins after 24 h of exposure to 5mm GalNAcα-O-benzyl. This effect was accompanied by an increased reactivity of these mucins to peanut lectin, testifying to the higher amount of T-antigen. The second observation was a decrease in the secretion of the mucins by GalNAcα-O-benzyl treated cells. The decrease in mucin sialyation was achieved through thein situ β-galactosylation of GalNAcα-O-benzyl into Galβ1–3GalNAcα-O-benzyl, which acts as a competitive substrate of Galβ1–3GalNAc α2,3-sialyltransferase, as shown by the intracellular accumulation of NeuAcα2–3Galβ1–3GalNAcα-O-benzyl in treated cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00702335
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  • 2
    Electronic Resource
    Electronic Resource
    Multiplex RT-PCR method for the analysis of the expression of human sialyltransferases: application to breast cancer cells (1998)
    Springer
    Glycoconjugate journal 15 (1998), S. 19-27 
    Details
    ISSN: 1573-4986
    Keywords: sialyltransferases ; breast cancer cells ; multiplex RT-PCR ; glycosyltransferases ; bp, base pair ; GAPDH, glyceraldehyde-3-phosphate dehydrogenase ; kb, kilobase ; PNA, Peanut (Arachis hypogaea) agglutinin ; RT-PCR, reverse transcription-polymerase chain reaction ; TBE, Tris base 0.13 M, boric acid 45 mM ; Na2EDTA 2.55 mM, pH 8.8 buffer ; Tm, melting temperature ; the nomenclature of sialyltransferases is based on that of Tsuji et al. [36]: ST3Gal I: CMP-NeuAc, Gal beta 1-3GalNAc alpha 2,3-sialyltransferase, EC 2.4.99.4 ; ST3Gal III: CMP-NeuAc, Gal beta 1-3/4GlcNAc alpha 2,3-sialyltransferase, EC 2.4.99.6 ; ST3Gal IV: CMP-NeuAc, Gal beta 1-4GlcNAc or Gal beta 1-3GalNAc alpha 2,3-sialyltransferase, EC 2.4.99. ; ST6Gal I: CMP-NeuAc, Gal beta 1-4GlcNAc alpha 2,6-sialyltransferase, EC 2.4.99.1.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In many cases of human cancer, the appearance of hypersialylated glycan structures is related to a precise stage of the disease; this may depend on altered regulation of one or more sialyltransferases genes. Since several distinct sialyltransferase enzymes arising from different unique genes transfer sialic acid residues in the same linkage onto the same acceptor, it is impossible to precisely determine which enzyme is involved in the observed phenotype based on enzymatic assays. We have developed a very sensitive and highly reproducible multiplex reverse transcriptase-polymerase chain reaction technique in order to monitor the expression of four human sialyltransferases genes ST6Gal I, ST3Gal I, ST3Gal III and ST3Gal IV in small cell samples. Multiplex PCR amplification using specific primers for each sialyltransferase and detection of amplification products by polyacrylamide gel electrophoresis is a method that is fast and easy to handle and has proven to be useful for establishing sialyltransferase patterns of expression in breast immortalized cell line HBL100 as well as in breast cancer cell lines MCF-7/6, MCF-7/AZ and MDA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006983214918
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    Paper (German National Licenses)
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