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  • 1
    Digitale Medien
    Digitale Medien
    The Effects of Hyperbaric Exposure on Human Peripheral Blood Mononuclear Cells, with Special Emphasis on Natural Killer Cell Cytotoxicity and Subsets (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Scandinavian journal of immunology 59 (2004), S. 0 
    Details
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Materials and methods:  As an experimental physiological stress model, we examined the effects of hyperbaric exposure on peripheral blood mononuclear cells (PBMCs) obtained from venous blood drawn from eight divers during a simulated heliox saturation dive. Eight persons working in normobar atmosphere outside the pressurized chamber served as control donors. The spontaneous cytotoxicity of the PBMCs was estimated in a 4 h 51Cr-release assay using k562 as NK-sensitive target cells. The PBMCs were characterized, using 4-colour flow cytometry, with special emphasis on the NK-cell subsets. The data were statistically analysed using a multivariate regression model (Stata 8.2). P values 〈0.05 was considered statistically significant.Results:  The estimated cytotoxicity increased significantly in both the group of divers and control donors during the dive (pdivers 〈 0.01 and pcontrols 〈 0.01). Although the cytotoxicity increased relatively more (P 〈 0.01) in the group of divers compared to the group of control donors between day 1 and 2.Discussion:  The increased cytotoxicity of PBMC estimated in the group of divers indicate that parts of the cellular immune system are affected during the extreme physiological conditions induced during the initial phase of the presented experimental hyperbaric setup. The increase in cytotoxicity observed in the group of control donors could hypothetically reflect the stress level in persons working outside the pressurized chamber during the dive.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01423aa.x
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  • 2
    Digitale Medien
    Digitale Medien
    Natural Killer Cell Functions and Subsets After In Vitro Stimulation with IL-2 and IL-12, with Special Emphasis on Intracellular IFN-γ and NK-Cell Cytotoxicity (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Scandinavian journal of immunology 59 (2004), S. 0 
    Details
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Materials and methods:  Isolated cryopreserved human peripheral blood mononuclear cells (PBMCs) were stimulated with IL-2 and IL-12. This stimulation has previously been shown to activate NK cells. Cell cytotoxicity was measured by flow cytometry after incubation with k562 cells. This method was compared to the current standard 51Cr release assay. Cells were treated with BFA to accumulate IFN-γ, stained for surface markers, permeabilized and stained for intracellular IFN-γ. Flow cytometry was then performed to measure intracellular IFN-γ production in PBMC, especially in NK cells.Results:  We have demonstrated that stimulation with IL-2 and IL-12 is effective in increasing the number of IFN-γ-positive cells. There is a distinct difference between the CD3-CD56dim and the CD3-CD56bright subsets, with a much greater proportion of IFN-γ-positive cells in the CD3-CD56bright subset. The effects of stimulation with IL-2 and IL-12 on cytotoxicity will be presented, as will the relation between IFN-γ production and cytotoxicity. In addition, we will present results of these assays applied to porcine cells.Discussion:  In combination, these tests will address NK cell function by combining cytotoxicity with IFN-γ production in NK cell subsets. The results will demonstrate whether this could serve as a useful tool in describing NK-cell function, which could be of value in clinical and experimental settings.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01423y.x
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  • 3
    Digitale Medien
    Digitale Medien
    Development of Bradycardia during Submersion of the Duck (1963)
    [s.l.] : Nature Publishing Group
    Nature 200 (1963), S. 684-685 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The receptor sites and the quality of the stimuli which bring forth the adjustments observed during submersion are unknown. It has been alleged that the actual immersion under water must play a dominant part in the afferent reflex chain, and that naturally diving animals are endowed with special ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/200684b0
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  • 4
    Digitale Medien
    Digitale Medien
    Renal Oxygen Tension (1960)
    [s.l.] : Nature Publishing Group
    Nature 188 (1960), S. 671-671 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Healthy dogs, weighing 15—28 kgm., were anaesthetized by ‘Nembutal’, 25 mgm./kgm. body-weight, and one kidney exposed by a flank incision. No water was given on the day of experiment, and the dogs were accordingly in the antidiuretic state. Available oxygen was determined by a 0-4-mm. thick ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/188671a0
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  • 5
    Digitale Medien
    Digitale Medien
    Heart rate in resting seals on land and in water
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 67 (1980), S. 77-83 
    Details
    ISSN: 0300-9629
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0300-9629(80)90410-7
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  • 6
    Digitale Medien
    Digitale Medien
    Seasonal changes in levels of growth hormone, somatomedin and thyroxine in free-ranging, semi-domesticated norwegian reindeer [Rangifer tarandus tarandus (L.)]
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 60 (1978), S. 123-126 
    Details
    ISSN: 0300-9629
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0300-9629(78)90215-3
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  • 7
    Digitale Medien
    Digitale Medien
    Insulation of furs in water
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part A: Physiology 47 (1974), S. 403-410 
    Details
    ISSN: 0300-9629
    Schlagwort(e): Insulation ; Pagophilus groenlandicus ; Ursus maritimus ; conductance ; fur ; temperature
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0300-9629(74)90002-4
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  • 8
    Digitale Medien
    Digitale Medien
    Vasomotor Responses in the Growing Antlers of the Reindeer, Rangifer tarandus (1969)
    [s.l.] : Nature Publishing Group
    Nature 223 (1969), S. 99-100 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Fig. 1. The two upper tracings demonstrate the increase in vascular resistance in reindeer antlers after injection of single doses of nor-adrenaline (10 µg) and histamine (4 µg) into the perfusion circuit. In reindeer legs (the two lower tracings), the responses to similar doses of ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/223099a0
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  • 9
    Digitale Medien
    Digitale Medien
    The effect of CVVHD and endotoxin on the oxidative burst, adhesion molecules and distribution in tissues of granulocytes (2000)
    Springer
    Intensive care medicine 26 (2000), S. 770-775 
    Details
    ISSN: 1432-1238
    Schlagwort(e): Key words Adhesion molecules ; CVVHD ; Endotoxin ; Granulocytes ; Oxidative burst
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Objective: Extracorporeal circulation, such as cardiopulmonary bypass and haemodialysis, has been associated with an activation of the immune system, especially the granulocytes. Continuous veno-venous haemodiafiltration (CVVHD) is used in critically ill septic patients. During CVVHD cytokines are excreted in the ultrafiltrate. But when the membranes used in CVVHD are cultured with granulocytes, the granulocytes are slightly activated. This effect is potentiated by endotoxin. We therefore, in vivo, compared the effect on granulocyte activation of CVVHD with an endotoxin group and a control group.¶Methods: Thirty-one pigs were anaesthetized and mechanically ventilated. In ten pigs CVVHD was performed. Eleven pigs received an infusion of Escherichia coli endotoxin 30 μ/kg–1 and ten pigs served as a control group. The adhesion molecules CD18 and CD62L were measured using monoclonal antibodies. The oxidative burst activity was assayed as superoxide dismutase-inhibitory reduction of cytochrome c. The number of granulocytes in peripheral blood and in the lungs and liver were counted.¶Results: The infusion of endotoxin was followed by granulocytopenia, reduced oxidative burst activity, increased expression of CD18 and decreased expression of CD62L on granulocytes. Accumulation of granulocytes in liver and lung tissue was also noted in this group. CVVHD was only associated with a non-significant decrease in CD62L expression on granulocytes. It did not affect any of the other measured immunological parameters.¶Conclusion: In contrast to endotoxin-induced sepsis, the granulocytes were not activated during CVVHD.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s001340051245
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