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  • 1
    Electronic Resource
    Electronic Resource
    HLA-D/DR Restriction of Macrophage-dependent Antigen Activation of Immune T Lymphocytes: Cross-reacting Allogeneic HLA-D/DR May Partly Substitute for Self HLA-D/DR (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Optimal proliferative response of T lymphocytes to purified protein derivative of tuberculin (PPD) III vitro requires that antigen be presented by autologous macrophages or allogeneic macrophages sharing HLA-D/DR determinants with the T cell donor. In some cases, however, T cells may respond to a limited extent to PPD in association with macrophages expressing different HLA-D/DR determinants. In this paper experiments are presented where various combinations of T cells and HLA-D/DR disparate macrophages were stimulated with PPD. We often found a stronger PPD-specific response in HLA-D/DR-incompatible combinations in which the macrophages carried HLA-DR antigens known from serological studies to be cross-reactive with those of the T cell donor than in combinations in which this was not the case. Thus, the cross-reactions detected by serology may sometimes be reflected on a functional level in T lymphocyte/macrophage cooperation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00022.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of Major Histocompatibility Complex Class II Subregion Products by Jejunal Epithelium in Patients with Coeliac Disease (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The MHC class II subregion product (HLA-DR), HLA-DP, and HLA-DQ) were located by immunofluorescence in serial sections of ethanol-fixed, paraffin-embedded jejunal mucosa from control subjects and patients with coeliac disease (CD). DR staining was seen in a granular luminal distribution and basolaterally on surface epithelial was positive for DR almost to the bottom of the glands. This contrasted with virtually absent glandular DR standing in controls and weak staining including only the upper part of the crypts in 5 out of 11 treated patients. HLA-DQ appeared only in three untreated patients and was restricted to patches of surface epithelium. The number of intraepithelial T lymphocytes per millimetre of surface epithelium was significantly higher in untreated than in treated CD patients or controls; it was also significantly higher in specimens with epithelial DP expression than in those without. This suggested that intraepithelial lymphocytes modulate epithelial class II expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02290.x
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  • 3
    Electronic Resource
    Electronic Resource
    Specificity of Two Subsets of Cytotoxic Human γδ T-Cell Clones (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 32 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Peripheral blood mononuclear cells were enriched for γδ T cells by immunomagnetic separation, stimulated with cells from an allogeneic donor, and cloned. T-lymphocyte clones (TLC) of the two major γδ T-cell subsets, BB3− (i.e. Vδ2+)and δTCSI+ (i.e. Vδ1/(D)/Jδ1). were obtained. In addition, one γδ TLC was BB3−δTCSI+. All of the BB3+ TLC showed strong cytotoxicity against various allogeneic tumour cell lines, such as Daudi and K562. The cytotoxicity against the tumour cell lines was modulated by MoAb against the γδ TcR. The BB3+ TLC were not cytotoxic against B-lymphoblastoid cell lines (B-LCL). In contrast, the δTCSI+ TLC showed much lower cytotoxic activity against the tumour cell lines, but many were strongly cytotoxic against allogeneic B-LCL. Some of the δTCSI + TLC demonstrated HLA-specific cytotoxicity, while other δTCSI+ TLC had a more broad cytolytic activity against B-LCL. Thus, the two major subtypes of γδ T cells from this donor, as defined by MoAb BB3 and δTCSI, were distinct with respect to recognition specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02902.x
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of in Vivo Hydrocortisone on Some Human Blood Lymphocyte Subpopulations (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 13 (1981), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of in vivo hydrocortisone (OHC) on natural killer (NK) activity was studied using the K562 cell line as target in a 3-h 51Cr-release assay. Peripheral blood lymphocytes obtained from live normal volunteers at 0, 4, 24 and 48 h after Intravenous administration of 300 mg of OHC showed significantly increased NK activity at 4 h, decreased activity at 24 h, with a return toward normal at 48 h. Parallel variation were found in the fraction of lymphocytes bearing receptor for the Fe part of IgG. However, neither the number of these cell nor the NK activity was influenced by the medication when the results were given per millilitre of blood. In vitro pre-incubation of the effector with OHC for 24 h had no effect on viability, expression of surface markers, or NK activity. It is concluded that under the present conditions NK activity is OHC-resistant. The variations observed after in vivo administration seem to be due to a reversible redistribution mainly affecting cells other than the NK effectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1981.tb00171.x
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on Human Epidermal Langerhans Cells (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Human epidermis was separated from dermis by means of a suction blister device and dissociated with trypsin. The epidermal cell suspensions obtained contained 3–5% Langerhans cells as judged by immunofluorescence staining of the cells with a rabbit anti-DR antiserum. The epidermal cells were co-cultured with purified allogeneic T cells and with autologous T cells with or without PPD of tuberculin. A strong T-cell response to allogeneic epidermal cells was obtained, as was a strong T-cell response to PPD, provided autologous epidermal cells were also present. Pre-treatment of the epidermal cells with anti-DR antiserum plus complement abolished both these responses. These data indicate that epidermal cells are able to substitute for macrophages both in the allo-activating and in the antigen-presenting function. Since the responsible cells were DR-positive, it is highly probable that the cells responsible for these functions are the Langerhans cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00006.x
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  • 6
    Electronic Resource
    Electronic Resource
    HLA-D-restricted Antigen Activation of Sensitized T Lymphocytes: Studies on the Ability of HLA-D/DR-expressing B Lymphocytes to Substitute for Macrophages in Antigen Activation (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 10 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The proliferative response of sensitized human T lymphocytes to purified protein derivative (PPD) and to trinitrophenyl (TNP)-conjugated autologous cells in vitro is restricted by self HLA-D/DR determinants. Here we report that the PPD-specific response is strictly related to the content of phagocytosing celts (macrophages, M) in the cultures and that an optinal PPD response occurred at a T/M ratio between 10:1 and 5:1. B-cell-enriched suspensions, which also express the HLA-D/DR determinants, were not able to replace the macrophages in this HLA-D/DR-restricted response. On the other hand, TNP-trealed similarly prepared B cells were in most instances effective in inducing a secondary TNP-specific response of in vitro-sensitized T cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb01349.x
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  • 7
    Electronic Resource
    Electronic Resource
    Gliadin Specific, HLA DQ2-Restricted T Cells are Commonly Found in Small Intestinal Biopsies from Coeliac Disease Patients, but not from Controls (1997)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 46 (1997), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The authors have analysed gliadin specific, CD4+ T cells isolated from small intestinal biopsies of 23 adult coeliac disease patients (20 on a gluten-free diet and three untreated) and nine control patients. The biopsies were stimulated ex vivo with a peptic/tryptic digest of gliadin for 24 h, and activated T cells were positively selected with paramagnetic beads coated with an antibody against the interleukin-2 receptor. The T cells were expanded and tested for gliadin reactivity and HLA restriction. Gliadin specific, polyclonal T cell lines were recovered from biopsies of all 23 patients. Inhibition studies of T cell lines from 21 patients with anti-HLA monoclonal antibodies indicated predominant presentation of the gliadin antigen by HLA-DQ2 in T cell lines from 11 patients (lines from seven patients with complete MoAb inhibition, the remaining with incomplete inhibition) and incomplete inhibition by HLA-DR3 in lines from three patients. Nine gliadin specific T cell clones from six patients were established; all of these were HLA-DQ2 restricted. Gliadin specific T cells were not found in biopsies from the non-coeliac controls. Our findings demonstrate that gliadin reactive T cells are commonly found in the intestinal mucosa of CD patients and they support the notion that the majority of T cells recognize gliadin peptide(s) when presented by the disease associated DQ2 molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.1996.d01-17.x
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  • 8
    Electronic Resource
    Electronic Resource
    Macrophage/T-Lymphocyte Interaction in the Immune Response to PPD in Humans (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 9 (1979), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The proliferative response of sensitized human T cells to low concentrations of purified protein derivative of tuberculin (PPD) in vitro has previously been shown lo depend on the presence of autologous or HLA-D-compatible macrophages (M). In this paper we present evidence that the M/T cell interaction requires viable M and docs not involve a soluble factor. T cells from neonates do not respond to PPD in our system, while M) from neonates are able 10 present PPD to T cells from HLA-D matched sensitized adults. It is also demonstrated that the previously reported HLA-D restriction of T cell/M9 cooperation is not due to T cell mediated killing of allogeneic macrophages in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1979.tb03279.x
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  • 9
    Electronic Resource
    Electronic Resource
    Possible Detection of HLA-DR Alloantigenic Specificities in Man with Unabsorbed Rabbit Antisera (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 8 (1978), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Thirty-six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA-D-homozygous cells and a rabbit antiserum to human B cells (anti-p 23/30). Thirty-one of the rabbits produced B-cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA-D (DR) antigen. Thus immunizazation of rabbits might prove valuable for the production of HLA-DR typing reagents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1978.tb00491.x
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  • 10
    Electronic Resource
    Electronic Resource
    Human Ia-Like Antigens Associated with HLA-D (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Antisera raised within HLA-A- and -B-compatible, HLA-D-disparate combinations were cytotoxic to B lymphocytes from the immunizing donor and to B lymphocytes from most or all cell donors sharing the immunizing donor's HLA-D phenotype. Four antisera recognized structures closely associated with the HLA-D determinants Dw2, Dw3, Dw4, and LD 108. One antiserum had a broad reactivity pattern, including Dw3, Dw6, and some unknown specificity(ies). In population and family studies these B-lymphocyte antigens behaved as if they were governed by one genetic locus in the B-D region of the HLA complex. Furthermore, the antisera were cytotoxic to a minor concanavalin-A-reactive T-cell subpopulation. The antisera had previously been shown to inhibit the stimulating cells in mixed lymphocyte culture and to be capable of inhibiting the Fc receptor in the EA rosette assay. We conclude that the antisera produced by this method recognize Ia-like antigens closely associated with the HLA-D determinants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02098.x
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