ISSN:
1572-9931
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Abstract We have analyzed the pattern of methylation of rearranged and germ line C K genes in DNA samples from human B-type chronic lymphatic leukemia lymphocytes and normal fibroblasts, and from granulocytes, T-, and non-T-lymphocytes separated from normal blood. C K alleles are flanked by one Hpall site on each side. Leukemic B-CLL DNA from five patients showed demethylation of these two sites in the productively rearranged allele and methylation of both sites in the nonexpressed allele whether rearranged or in germline configuration. Non-T-lymphocytes from normal individuals also showed part of the C K genes to have demethylation of both Hpall sites. On the other hand, C K genes from normal granulocytes, Tlymphocytes, and four of five fibroblast samples were methylated in one or both Hpall sites. We propose that in Blymphocytes, demethylation of C K alleles is a specific event taking place in the expressed genes and being associated with an increased transcriptional activity of the gene. In addition to this specific demethylation, there is also an unspecific one that may appear in cells with silent C K genes and that do not modify the expression of the gene.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01535045
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