ISSN:
1432-1424
Schlagwort(e):
Alpha toxin
;
Channel size
;
Conductance states
;
Planar bilayer
;
Polymer exclusion
;
Staphylococcus aureus
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract We have used a polymer-exclusion method to estimate the sizes of the high and low-conductance states of Staphylococcus aureus α-toxin channels across planar lipid bilayers. Despite a 〉10-fold difference in conductance between high and low-conductance states, the size differs by 〈2-fold. We conclude that factors other than the dimensions have a strong influence on the conductance of α-toxin channels. We also show that the high conductance state is destabilized by the presence of high molecular weight polymers outside the channel, compatible with the removal of channel water as the high conductance state “shrinks” to the low conductance state.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00234521
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