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  • 1
    Digitale Medien
    Digitale Medien
    Cellular location of peroxidase isoenzymes in leaf tissue of Petunia and their affinity for Concanavalin A-Sepharose (1985)
    Springer
    Planta 164 (1985), S. 89-95 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Concanavalin A ; Leaf (peroxidases) ; Peroxidase isoenzymes ; Petunia (peroxidases)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The cellular location of three peroxidase isoenzymes (PRX) in mature leaf tissue of Petunia and their affinity for Concanavalin A-Sepharose were investigated. The isoenzymes PRXa, PRXb and PRXc were identified by their positions in starch-gel zymograms. The fast-moving anodic and cathodic peroxidase bands, the isoenzymes PRXa and PRXc respectively, were the most active peroxidases in extracellular extracts. The molecular forms of PRXa showed a tissue-specific distribution between midrib and remaining leaf tissue. An intermediate-moving anodic peroxidase band, the isoenzyme PRXb, was the most active peroxidase released after extraction of isolated mesophyll protoplasts. Small amounts of the peroxidase isoenzymes were present in cell-wall-bound fractions. Incubation of a crude protein fraction with Concanavalin A-Sepharose showed that the isoenzyme PRXb bound more firmly to Concanavalin A-Sepharose than the isoenzymes PRXa and PRXc, of which only one molecular form bound partly. The results are discussed with respect to a possible function of one of the peroxidase isoenzymes, and a possible role of oligosaccharide chains in determining the cellular location of plant peroxidases is suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00391030
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  • 2
    Digitale Medien
    Digitale Medien
    Immunological studies on lysosomal sphingomyelinase: Identification of a 28 000-Da component deficient in urine from patients with Niemann-Pick disease types A and B (1984)
    Springer
    Bioscience reports 4 (1984), S. 1051-1057 
    Details
    ISSN: 1573-4935
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The immunoblotting technique was used to identify sphingomyeJinase protein in samples of tissue and urine after subjection to poIyacrylamide-gel etectrophoresis in the presence of sodium dodecyl sulphate. In a sphingomyelinase preparation purified from control urine a prominent band was seen with an Mr of 28 000 Da. Glycoprotein fractions from urine and placenta, a membrane extract from spleen, and a partially purified sphingomyelinase preparation from placenta contained the 28 000-Da band plus additional, higher-Mr bands. The 28 000-Da band was detectable in urine from a patient with Niemann-Pick disease type C, but not in urine from patients with Niemann-Pick disease types A and B. It is concluded t h a t sphingomyeJinase is composed of at least one polypeptide with an Mr of 28 000 Da and that this polypeptide is deficient in the urine of patients with Niemann-Pick disease types A and B.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01116698
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  • 3
    Digitale Medien
    Digitale Medien
    Resistance to tomato spotted wilt virus in transgenic tomato hybrids (1955)
    Springer
    Euphytica 85 (1955), S. 159-168 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Lycopersicon ; tomato ; tomato spotted wilt virus ; tospovirus ; transformation ; virus resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Tomato spotted wilt virus (TSWV) causes significant economic losses in the commercial culture of tomato (Lycopersicon esculentum Mill.). Culture practices and introgression of natural sources of resistance to TSWV have only been marginally effective in controlling the TSWV disease. Recently however, high levels of protection against TSWV have been obtained by transforming tobacco with a chimaeric gene cassette comprising the TSWV nucleoprotein gene. This report demonstrates the successful application of this newly-created TSWV resistance gene in cultivated tomato. Transformation of an inbred tomato line with the TSWV nucleoprotein gene cassette resulted in high levels of resistance to TSWV that were maintained in hybrids derived from the parental tomato line. Therefore, transformant lines carrying the synthetic TSWV resistance gene make suitable progenitors for TSWV resistance to be incorporated into the breeding programmes of tomato.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023944
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  • 4
    Digitale Medien
    Digitale Medien
    Regulation of UFGT activity in the bz-m4 allele of Zea mays: A possible case of gene fusion (1983)
    Springer
    Molecular genetics and genomics 190 (1983), S. 1-4 
    Details
    ISSN: 1617-4623
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Revertant derivates (bz-m4 to Bz′ 1 and Bz′ 7) of the bz-m4 sh mutant allele were investigated for their UDP-Glucose: flavonoid 3-O-glucosyltransferase (UFGT) activity in the subaleurone endosperm layer of developing kernels. The UFGT activity profile during development in Bz′ 1 subaleurone endosperm is partly the same as that of the bz-m4 mutant. This result is interpreted as supportive evidence for the occurence of gene fusion in the original bz-m4 sh mutant allele, causing the alteration in time and place of activity of the structurally unaltered UFGT in bz-m4 kernels. The possibility that regulating sequences of the sh gene are fused with the structural sequences of the Bz gene is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00330316
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