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Digitale Medien

Stimulation of partial development of human mast cells by supernatant fluid from mouse fibroblast cultures (1994)

DVORAK, A. M. ; MITSUI, H. ; ISHIZAKA, T.

Oxford, UK : Blackwell Publishing Ltd

Clinical & experimental allergy 24 (1994), S. 0

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Details

ISSN: 1365-2222

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Summary. Fibroblasts have been implicated as culture-competent cells for the mast cell lineage in several species. In man, fibroblast monolayers sustain the ultrastructural phenotype and function of isolated human lung mast cells and permit the differentiation and full maturation of human mast cells from their agranular precursors in cord blood cells. We examined whether development and maturation of the mast cell lineage in man can be achieved by a supply of the soluble products present in fibroblast culture supernatants. Suspension cultures of cord blood cells were supplemented with culture supernatants derived from two different murine fibroblast lines; controls were not supplemented. The cultures were sampled for light and electron microscopy at 6, 7 and 8 weeks. Human mast cells developed in quantity when cultures were supplemented with the supernatants from BALB/c 3T3 fibroblasts, in reduced numbers when supplemented with Swiss Albino 3T3 fibroblast supernatants, and not at all in culture media alone. By ultrastructural criteria, the newly developed mast cells did not achieve full maturity; they did continue to synthesize new granules and to undergo intragranular maturational events. Small numbers of mature basophils persisted in suspension cultures, and many were undergoing piecemeal degranulation. Other cell lineages noted included eosinophils, neutrophils, macrophages and endothelial cells. We conclude that a factor(s) of fibroblast origin permits the differentiation and partial maturation of human mast cells from their agranular precursors in cord blood, but that fibroblasts must be physically present for complete maturation of these lineages to occur.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1365-2222.1994.tb00969.x

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Digitale Medien

Identification of a gene family (kat) encoding kinesin-like proteins in Arabidopsis thaliana and the characterization of secondary structure of KatA (1993)

Mitsui, H. ; Yamaguchi-Shinozaki, K. ; Shinozaki, K. ; [weitere]

Springer

Molecular genetics and genomics 238 (1993), S. 362-368

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Details

ISSN: 1617-4623

Schlagwort(e): Kinesin-related gene ; Gene family ; Arabidopsis thaliana ; Nucleotide sequence ; Secondary structure

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract A gene family, designated kat, has been characterized in Arabidopsis thaliana by genomic Southern hybridization and nucleotide sequencing analysis. The kat gene family includes at least five members, named katA, katB, katC, katD and katE, whose products share appreciable sequence similarities in their presumptive ATP-binding and microtubule-binding motifs with known kinesin-like proteins. The carboxyl-terminal region of the KatA protein deduced from the nucleotide sequence of the cDNA clone has considerable homology with the mechanochemical motor domain of the kinesin heavy chain. The predicted secondary structure of the KatA protein indicates two globular domains separated by a long a helical coiled coil with heptad repeat structures, such as are commonly found in kinesin-like proteins.