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Digitale Medien

COLLAGENASE PRODUCTION BY HUMAN SYNOVIAL TISSUES (1975)

Krane, Stephen M.

Oxford, UK : Blackwell Publishing Ltd

Annals of the New York Academy of Sciences 256 (1975), S. 0

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Details

ISSN: 1749-6632

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Allgemeine Naturwissenschaft

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1749-6632.1975.tb36056.x

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Digitale Medien

Regulation of hormone-induced cyclic AMP response to parathyroid hormone and prostaglandin E2 in cells cultured from human giant cell tumors of bone (1979)

Goldring, Steven R. ; Dayer, Jean-Michel ; Krane, Stephen M.

Springer

Calcified tissue international 29 (1979), S. 193-200

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ISSN: 1432-0827

Schlagwort(e): cyclic AMP ; Parathyroid hormone ; Prostaglandin E2 ; Down regulation ; Bone cells

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Physik

Notizen: Summary Cells dispersed from human giant cell tumors of bone and grown in monolayer culture increase intracellular cyclic AMP (cAMP) when incubated with parathyroid hormone (PTH) or prostaglandin E2 (PGE2). When cells are continuously exposed to PTH, cAMP levels increase acutely but then decrease rapidly to pretreatment values despite continued presence of hormone or addition of new hormone. Preincubation of cells with PTH for periods as short as 10 min results in a decrease in the capacity of cells to increase cAMP content when re-exposed to maximal stimulatory concentrations of PTH. The decrease in the magnitude of the PTH-induced cAMP response observed in cells pretreated with this hormone is dependent on the concentration of PTH present during the pre-incubation. The loss of cAMP response in cells pre-treated with either PGE2 or PTH is hormone specific in that cells made refractory by pretreatment with one hormone still increase cAMP content when exposed to the other. Although the cells are not releasing measurable amounts of prostaglandins into the medium, pretreatment with indomethacin results in an increase in the magnitude of the cAMP response to PGE2. The PTH-induced cAMP response is not affected by indomethacin pre-treatment. The loss of PTH responsiveness produced by hormone preincubation is consistent with the phenomenon of “down-regulation” observed with ligand-receptor interactions in a variety of tissues.