ISSN:
1432-1424
Schlagwort(e):
Na+/K+
;
ATPase
;
cDNA expression
;
Subunit assembly
;
Xenopus oocyte
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract The cRNA for Torpedo californica Na+/K+-ATPase β-subunit (cRNAβ) was injected into Xenopus oocytes alone or with the cRNA for the Na+/K+-ATPase α-subunit (cRNAα). When cRNAβ was injected alone, the amount of the β-subunit that accumulated in oocytes increased with increasing amounts of injected cRNAβ. When cRNAβ and cRNAα were injected simultaneously, less β-subunit accumulated than when cRNAβ was injected alone, whereas the Na+/K+-ATPase activity increased markedly. The decrease in the accumulation of the β-subunit was dose-dependent upon the cRNAα. The mutant β-subunit unable to assemble with the α-subunit accumulated in oocytes independently of cRNAα, suggesting that post-translational control mechanisms may serve to reduce the accumulation of the β-subunit.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00234155
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