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Digitale Medien

Effects of some factors on protoplast formation of a microalga, Prototheca zopfii (1997)

Suzuki, T. ; Yamaguchi, T. ; Ishida, M.

Springer

World journal of microbiology and biotechnology 13 (1997), S. 355-356

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ISSN: 1573-0972

Schlagwort(e): Achlorophyllous alga ; enzymatic degradation ; protoplast formation ; Prototheca.

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The effects of experimental factors on protoplast formation of Prototheca zopfii Kru¨ger in 0.85 m NaCl using Macerozyme R-200 were studied based on a fractional factorial experimental design. The rate of protoplast formation was mainly affected by the incubation temperature and the age of algal cells. The optimal condition for the maximum protoplast yield was determined based on a response surface model. These were: mid-logarithmic phase cells and Macerozyme concentration of 4% at a temperature of 35°C.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1018555628895

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