ISSN:
1612-1112
Schlagwort(e):
Thin-layer chromatography
;
Monophenyl-treated thin layer plate
;
Phenyldimethylethoxysilane treated plate
;
Flavonoids inScutellariae radix
;
Baicalin
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The retention and selectivity of flavonoids (baicalin, baicalein, wogonin, oroxylin A) inScutellariae radix have been studies by high-performance thin-layer chromatography on phenyldimethylethoxysilane-treated silica plates. The silica plates treated with phenyl groups were used for physical and chemical analysis. From elemental carbon analysis, the maximum number of bonded phenyl surface groups per gram was calculated to be 0.467×1021 (Oginal silica plate: Merck Art. 15109, Silica gel 100 F254). With methanol-1/15 M phosphate buffer (pH 6.2) mixtures as mobile phase, baicalin, baicalein, wogonin, and oroxylin A inScutellariae radix were separated. It has been shown that phenyl-treated plates are more suitable for selective separation of baicalin, baicalein, wogonin, and oroxylin A than octadecyl-treated plates.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02277510
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