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Digitale Medien

A simple method of fast extracellular solution exchange for the study of whole-cell or single channel currents using patch-clamp technique (1987)

Hering, S. ; Beech, D. J. ; Bolton, T. B.

Springer

Pflügers Archiv 410 (1987), S. 335-337

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Details

ISSN: 1432-2013

Schlagwort(e): Concentration-jump technique ; Patch-clamp technique ; Single smooth muscle cell

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract A new concentration-jump technique was devised for the rapid application of drugs to single, isolated cells attached to the base of the experimental chamber while recording from them with patch-clamp technique. Cells were placed in a micro-drop (less than 0.1 μl) in a small inner bath which was separated from an outer bath by a ring of “Sylgard” polymer. Stable whole-cell recordings were made in the micro-drop and rapid solution exchange took place when a much larger volume of test solution from the outer bath was flooded over the Sylgard ring and mixed with the micro-drop. Complete equilibration occurred within less than 10 ms.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00580285

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