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Digitale Medien

Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract (1994)

Tsai-Wu, Jyy-Jih ; Lu, A-Lien

Springer

Molecular genetics and genomics 244 (1994), S. 444-450

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Details

ISSN: 1617-4623

Schlagwort(e): Escherichia coli ; mutY ; DNA mismatch repair ; DNA polymerase ; Repair tract

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mufY-dependent repair tract was measured by the specific incorporation of α-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3′ side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00286698

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