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Digitale Medien

Quantification of total IgM and IgG levels in rat sera by a sandwich ELISA technique (1994)

Salauze, D. ; Serre, V. ; Perrin, C.

Springer

Comparative clinical pathology 4 (1994), S. 30-33

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Details

ISSN: 1433-2981

Schlagwort(e): Age ; ELISA ; IgG ; IgM ; Immunotoxicology ; Sprague-Dawley rats

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract Routine determination of IgG and IgM levels is likely to be included in future international immunotoxicity assessment guidelines with the rat as the required animal species. Only a few historical data are available on these two parameters in the rat. We describe a quantitative ELISA sandwich method using commercially available purified rat immunoglobulin as standards and provide the normal range for IgG and IgM values in healthy untreated 10 ± 2 to 30 ± 3 weekold Sprague-Dawley rats. Our data indicate that both IgM and IgG levels increase gradually with age. In addition, we determined IgG values in rats hyperimmunised with protamine, lysozyme and casein.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00368263

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