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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 75 (1988), S. 286-290 
    ISSN: 1432-2242
    Keywords: RFLP ; Wheat ; Aegilops ; Rye ; β-Amylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtained from wheat aneuploid and wheat-alien addition lines. Structural genes were identified on homoeologous group 4 and 5 chromosomes, confirming the results of isozyme studies. In addition, a further set of structural genes was found on homoeologous group 2 chromosomes. It is proposed that there are two homoeoallelic series, β-Amy-1 on group 4 or 5 chromosomes, and β-Amy-2 on group 2 chromosomes. Evidence is presented that each locus contains one or two β-amylase structural genes, and it is suggested that the large number of isozymes seen upon IEF are due to post-translational modifications.
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; RFLPs ; Homoeology ; Chromosome arm ; Alien additions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate the use of RFLP analysis in the Triticeae, a set of low copy number probes has been isolated from a wheat cDNA library. The probes identify each of the 14 homoeologous chromosome arms of wheat as determined by analysis of DNA fragments hybridizing to the probes in aneuploid lines of Chinese Spring. These probes can be used in RFLP analyses both for the assignment of homoeology of alien chromosomes or arms added to wheat, and for the determination of chromosome dosage in wheat aneuploids. Different chromosomes from various Triticeae species can therefore be followed in a wheat genetic background using a single technique. The potential uses of the set in facilitating the transfer of alien segments into wheat are outlined.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 691-699 
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; β-Amylase ; Isozymes ; RFLPs ; IEF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a β-amylase probe using three restriction enzymes, and for mature grain β-amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain β-amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 136-142 
    ISSN: 1432-2242
    Keywords: Drosophila melanogaster ; Genotype × Environment interaction ; Heterosis ; Epistasis ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between heterozygosity and the expression of heterosis at two different nutrition levels was investigated using Drosophila melanogaster. Average daily egg production and egg hatchability were measured in two parental strains and in F1, F2 and reciprocal backcross generations. Heterosis was more pronounced in the poor nutritional conditions. Two electrophoretic markers used to estimate the level of heterozygosity in F2 and backcrosses revealed an excess of heterozygous genotypes. Quantitative genetic effects (an additive line effect and individual and maternal heterosis) were estimated for both traits in the two environments. Although this model gave a reasonable fit in most cases, some epistatic interaction would have to be invoked in order to explain fully the results.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 78 (1989), S. 495-504 
    ISSN: 1432-2242
    Keywords: Wheat ; Genetic mapping ; RFLPs ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) mapping was attempted using 18 cDNA clones, 14 anonymous and 4 of known function, which had been shown to have homologous DNA sequences on the group 7 chromosomes of wheat. The loci identified by these probes have been mapped on one or more chromosomes in this homoeologous group using linkage data derived from various F2, random inbred, doubled haploid and single chromosome recombinant populations. The maps also include three isozyme loci, five disease resistance loci, two anthocyanin pigment loci and a vernalisation response locus. The mapping data have been used to determine the extent of map co-linearity over the A, B and D genomes, the degree of RFLP variability in the three genomes and the relative efficiency of various restriction enzymes in detecting RFLPs in wheat. The strategy for future mapping in wheat, particularly the use of “alien” genomes or segments, such as that from Aegilops ventricosa used here, is discussed.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 66 (1985), S. 145-150 
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chiasma frequency in spermatocytes was found to show extensive variation between individual males from a natural population of the marsupial Trichosurus vulpecula. Two factors which might contribute to the observed variation were investigated; season and age. However, neither of these had any significant effect on chiasma frequency or chiasma frequency variation.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 26 (1988), S. 57-61 
    ISSN: 1741-0444
    Keywords: Colour coding ; Image display ; Radionuclide imaging ; Uniform chromaticity scale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The use of digital technology in medical imaging gives the potential for improving the way in which images are displayed. One possibility is to represent intensity information by false colours rather than the conventional grey shades. Although this has several potential advantages, it has not been widely accepted. The aim of this study was to compare the performance of grey scale coding with three colour scales: the hot-body scale, a blue-green-red scale derived from the Uniform Chromaticity Scale, and a contrasting-colour scale. Two perceptual tasks were studied using computer-simulated images of the type produced in nuclear medicine. The first was to test the ability to perceive changes in image sharpness. The grey scale, hot-body scale and blue-green-red scale performed equally well but the contrasting-colour scale was significantly worse than others. The second pattern measured the ability to detect small changes in image intensity. The contrasting colour scale was best for this task and the grey scale worst. While the best colour scale depends on the information which is to be extracted from the image, both the hot-body scale and the blue-green-red scale appeared to offer a satisfactory compromise.
    Type of Medium: Electronic Resource
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  • 18
    ISSN: 1432-0878
    Keywords: Progesterone receptor ; Hypothalamus ; Forebrain ; Immunohistochemistry ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell nuclei containing progesterone receptor were identified immunohistochemically in the hypothalamus and forebrain of the domestic hen using an antiserum to the steroid binding “B” subunit (110 kDa) of chicken oviduct progesterone receptor and the avidin-biotin complex procedure. Cell nuclei containing progesterone receptor were widely distributed in the anterior, medial and basal hypothalamus with the highest density occurring in the lamina terminalis and the preoptic area. Abundant, though less intensely reacting progesterone receptor was present in cell nuclei in the tuberal infundibular area and in the internal zone of the median eminence. A large group of cell nuclei containing progesterone receptor occurred in the dorsal anterior hypothalamus between the anterior commissure and the lateral ventricle. This group of nuclei extended anteriorly into the telencephalon. A small number of cell nuclei containing progesterone receptor was also found in the ventral telencephalon in the region of the nucleus accumbens.
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 27 (1989), S. 277-280 
    ISSN: 1741-0444
    Keywords: Colour scales ; Combined images ; Fast image analysis ; Image processing ; Luminance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The interpretation of NMR images at Aberdeen is most often performed by the individual study of the proton density, difference, inversion recovery and T1 image. This report investigates the usefulness of presenting the information from a pair of images, in this instance the T1 and proton density images, in a single composite image. Information from the two images is combined such that the values from one image are represented by a change in hue (colour), and the values from the other by a change in luminance (intensity). To test the advantages of such combined images, a trial was run using a selection of prediagnosed abnormal brain scans. The information perceived as hue and luminance in the combined images was compared with that from separate conventional monochrome proton density and T1 images. Medical and nonmedical users were told the final diagnosis and were asked whether it was possible to see more, less, or the same information with regard to clinically relevant details in the structure of the abnormality and the image as whole. The results have revealed that for the majority of cases the combined image format can effectively represent the information normally contained in both the monochrome proton density and T1 images—thus speeding up the image viewing process.
    Type of Medium: Electronic Resource
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