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  • 11
    Electronic Resource
    Electronic Resource
    Cloning of the glucose isomerase (D-xylose isomerase) and xylulose kinase genes of Streptomyces violaceoniger (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 121-126 
    Details
    ISSN: 1617-4623
    Keywords: Streptomyces ; Glucose isomerase ; Xylose utilisation ; Recombinant plasmids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Xylose utilization mutants of Streptomyces violaceoniger were isolated lacking one or both of the enzymes, glucose isomerase (xylose isomerase) and xylulose kinase. Using pUT206 as a cloning vector, complementation of the glucose isomerase negative phenotype with fragments of the S. violaceoniger chromosome permitted isolation of two recombinant plasmids, designated pUT220 and pUT221, which contained 10.6 and 10.1 kb of chromosomal DNA, respectively. Both of these plasmids complemented all three different classes of xylose negative mutants and also provoked an increase of glucose isomerase and xylulose kinase activity in the mutant and wild-type strains. Plasmid pUT220 was chosen for detailed study by subcloning experiments. The putative glucose isomerase gene was localized to a 2.1 kb segment of the 10.6 kb chromosomal DNA fragment. The putative xylulose kinase gene resides nearby. Thus both genes seem to be clustered at a single chromosomal localization. This organization appears similar to that of the xylose utilization pathway in Escherichia coli, Salmonella typhimurium and Bacillus subtilis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330432
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  • 12
    Electronic Resource
    Electronic Resource
    Effects of phleomycin-induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 225-231 
    Details
    ISSN: 0749-503X
    Keywords: fission yeast ; cell cycle ; phleomycin ; DNA damage ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effect of phleomycin, a bleomycin-like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe. We report that in response to phleomycin-induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2-phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA-damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110305
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    Paper (German National Licenses)
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