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  • 1
    ISSN: 0009-8981
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Medizin
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    FEBS Letters 39 (1974), S. 200-204 
    ISSN: 0014-5793
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 87 (1979), S. 1-8 
    ISSN: 0006-291X
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    ISSN: 1569-8041
    Schlagwort(e): B-DLCL ; clinical correlations ; genetic lesions ; outcome
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background: B-diffuse large-cell lymphomas (DLCL) have been associated with some molecular lesions, but the role of such lesions as prognostic markers is still controversial. This report concerns an investigation of the frequency and clinical correlation of bcl-6, bcl-2, c-myc rearrangements and 6(q) deletions in B-DLCL. Patients and methods: The presence of these genetic lesions was analyzed in samples of lymph nodes or bone marrow collected at diagnosis in 71 patients with B-DLCL, all treated with an antracycline-containing chemotherapy regimen. Results: Rearrangement of bcl-6 was found in 11 patients (15%), rearranged bcl-2 in 12 (17%), 6(q) deletions in 10 patients (14%) and c-myc rearrangement in four (6%). Patients with rearranged bcl-6 tended to have a more aggressive disease than patients with germ-line bcl-6 (intermediate–high/high risk according to IPI criteria: 73% vs. 43%), but there were no differences in three-year survival rates (62% vs. 42%) between the two groups. The numbers of involved extranodal sites were similar in patients with rearranged and those with germ-line bcl-6. Patients with bcl-2 rearrangement appeared to have a less aggressive disease than those with germ-line bcl-2 (low/low–intermediate risk 75% vs. 47%) and a slightly better three-year survival rate (70% vs. 41%) but again the difference was not significant. Both groups with or without 6(q) deletion had similar clinical characteristics and outcomes. The four patients with c-myc rearrangement had aggressive disease and did poorly. Conclusions: The analysis of molecular lesions in B-DLCL may be useful for a better diagnostic definition; however, in this study we were unable to show that the evaluated genetic lesions had a significant impact on clinical outcome.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1432-0584
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Bei 10 gesunden Menschen und 10 Patienten mit Leberzirrhose wurde der Serumeisen-Umsatz, die Ferrokinetik im Organismus, die Hämoglobinsynthese, die Halbwertzeit der Erythrozyten und die mittlere Reifungszeit des Erythrons mit Hilfe von59Fe untersucht. Der Eisenumsatz und die Hämoglobinsynthese waren bei der Leberzirrhose zwar signifikant erhöht, jedoch nicht so ausreichend, daß dadurch die Reduktion der Erythrozytenlebensdauer kompensiert wird. Die mittlere Reifungszeit des Erythrons war normal. Nach den vorliegenden Befunden konnten zwei Faktoren für die Anämie bei Leberzirrhose verantwortlich gemacht werden: die Hämolyse und die relative Knochenmarksdepression. Mit Hilfe der angewandten Methodik war es möglich, die Rolle dieser Faktoren bei allen untersuchten Patienten quantitativ aufzudecken.
    Notizen: Summary Turnover of plasma iron, kinetics of iron through the body compartements, hemoglobin synthesis, red cell half life and mean effective erythron maturation time were studied, by using Fe59, in 10 normal subjects and 10 cirrhotic patients. Iron turnover and hemoglobin synthesis were significantly raised, but not sufficiently to compensate the red cell life span reduction. Mean effective erythron maturation time was normal. In conclusion two factor were responsible for the anemia: the hemolysis and a relative marrow depression. By the method used it was possible to discriminate quantitatively their importance in all the subjects investigated.
    Materialart: Digitale Medien
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  • 7
    ISSN: 1432-0584
    Schlagwort(e): Chronic myeloid leukemia ; Tumor suppressor genes ; Blastic transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We have investigated the involvement of the p53 and RB1 tumor-suppressor genes in 26 cases of chronic myeloid leukemia (CML) blast crisis, including 17 myeloid, eight lymphoid, and one megakaryoblastic crisis. The presence of p53 mutations in exons 5 through 9 was tested by the PCR-single-strand conformation polymorphism (SSCP) assay, followed by PCR-direct sequencing; in addition, loss of heterozygosity (LOH) at 17p13, the site of the p53 gene, was assayed by Southern blot. Given the variability of the mechanisms of inactivation of the RB1 gene in human tumors, a combination of Southern blot and mutational analysis by PCR-SSCP was used. p53 mutations were restricted to one case of myeloid blast crisis, showing a CGC→TGC (Arg→Cys) mutation at codon 283; two additional cases displayed LOH at 17p13 in the absence of p53 mutations. No molecular lesions of the RB1 gene were detected in any of the cases analyzed. These data indicate that inactivation of p53 and RB1 is a rare event in the molecular pathogenesis of CML acute transformation.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Annals of hematology 16 (1968), S. 333-341 
    ISSN: 1432-0584
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Ergebnisse von Untersuchungen zur Feststellung, ob die Höhe der Protoporphyrinsynthese aus δ-Aminolävulinsäure bei Eisenmangelanämie geringer ist und ob Eisen für die Synthese von Zwischenprodukten im Porphyrinstoffwechsel wesentlich ist, werden berichtet. Durch das Messen von δ-Aminolävulinsäure im Harn, Porphobilinogen-, Koproporphyrin- und Uroporphyrinausscheidung im Harn und die fäkale Ausscheidung von Koproporphyrin und Protoporphyrin wurde ein Absinken der Höhe der Porphyrinsynthese bei Fällen von idiopathischer hypochromer Anämie nachgewiesen. Der relative Anstieg in der Porphobilinogenausscheidung im Harn deutet auf eine Teilblockierung der Porphyrinsynthese von Porphobilinogen. Eisenbehandlung normalisiert den Porphyrinstoffwechsel.
    Notizen: Summary The results of studies done to ascertain whether the rate of protoporphyrin synthesis from δ-aminolaevulic acid is decreased in iron deficiency anaemia and whether iron is essential for the synthesis of the intermediate compounds in the porphyrin metabolism are reported. By measuring the urinary δ-amino-laevulic acid, porphobilinogen, coproporphyrin and uroporphyrin excretion and the faecal coproporphyrin and protoporphyrin excretion a decrease in the rate of porphyrin synthesis was demonstrated in cases of idiopathic hypochromic anaemia. The relative increase in the urinary porphobilinogen excretion points to a partial block of porphyrin synthesis from porphobilinogen. Iron treatment normalises the porphyrin metabolism.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Annals of hematology 17 (1968), S. 14-19 
    ISSN: 1432-0584
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Die Autoren berichtenüber die Ergebnisse von In-vitro-Untersuchungen über die Höhe der Porphobilinogen- und der Porphyrinsynthese in menschlichen Erythrozyten mit Eisenmangel. Es zeigte sich, 1. daß die Synthese von Porphobilinogen aus δ-Aminolacvulinsäure praktisch normal ist; 2. daß in Erythrozyten mit Eisenmangel die Aminolaevulinsäure dem gleichen Stoffwechselweg folgt wie in normalen roten Blutkörperchen; 3. daß die Synthese von Porphyrin aus Porphobilinogen zum Teil gehemmt und die Menge des gebildeten Porphyrins erheblich verringert ist; 4. daß die Gabe von Eisen die Höbe der Porphyrinsynthese aus Porphobilinogen normalisiert ohne die Synthese diese Pyrolls zu stören.
    Notizen: Summary The results ofin vitro studies on the rate of porphobilinogen and porphyrin synthesis in iron deficient human red blood cells are reported. It was found: 1. that the synthesis of porphobilinogen from δ-aminolaevulic acid is virtually normal; 2. that in iron deficient red blood cells δ-aminolaevulic acid follows the same metabolic paths as in normal red blood cells; 3. that the synthesis of porphyrin from porphobilinogen is partly inhibited, the amount of porphyrin formed being appreciably decreased; 4. that the administration of iron normalises the rate of porphyrin synthesis from porphobilinogen without interfering with the synthesis of this pyrrole.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1432-0584
    Schlagwort(e): Microsatellite instability ; Genomic instability ; Chronic lymphocytic leukemia ; Richter's syndrome ; Lymphoma
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Microsatellite instability (MSI) represents one specific pattern of genomic instability and is one of the genetic lesions most frequently detected in human neoplasia. Although MSI has been found to be associated with a wide variety of solid cancers, its involvement in lymphoid malignancies is virtually unexplored. In this study, we have investigated the presence of MSI in chronic lymphoproliferative disorders by comparing the pattern of nine microsatellite repeats (two tetranucleotides, two trinucleotides, and five dinucleotides) on autologous germline and tumor DNA of 23 patients, including 17 with B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL), four with hairy cell leukemia, one with lymphoplasmacytoid lymphoma, and one with T-cell chronic lymphocytic leukemia. All samples at diagnosis displayed a germline pattern of the microsatellites examined, thus suggesting that MSI is not involved in the pathogenesis of these lymphoproliferations. Also, no microsatellite alterations were observed in consecutive samples of B-CLL/SLL obtained from the same patient at various stages of the disease both before and after chemotherapy. Conversely, alterations in 3/9 microsatellite repeats were detected in one case of Richter's syndrome which had evolved from a pre-existent B-CLL/SLL phase. Overall, the low frequency of MSI among chronic lymphoproliferative disorders adds further weight to the common view that the mechanisms and patterns of genomic instability in lymphoid neoplasia differ markedly from those commonly observed in solid cancers.
    Materialart: Digitale Medien
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