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  • 1
    Electronic Resource
    Electronic Resource
    Neuronal Nicotinic Acetylcholine Receptors on Bovine Chromaffin Cells: Cloning, Expression, and Genomic Organization of Receptor Subunits (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 68 (1997), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Neuronal nicotinic acetylcholine receptors from bovine adrenomedullary chromaffin cells play a primary role in triggering catecholamine secretion. In the present study, their constituent subunits were characterized. In addition to the α3 subunit, which we have previously cloned, the presence of α5 and β4 but not of β2 subunits was detected by reverse transcription-PCR analysis of mRNA from adrenal medulla. In situ hybridization indicated that α3, α5, and β4 subunits are coexpressed in all chromaffin cells. The primary structure of α5 and β4 subunits was determined and functional receptors were obtained upon coinjection of subunit cRNAs into Xenopus oocytes. In contrast to other β4-containing nicotinic receptors, the ones formed by the bovine β4 subunit are insensitive to the agonist cytisine. Finally, we characterized the intergenic region of α3 and α5 subunits, which together with the β4 subunit, form a gene cluster in rats and chickens. RNase assays and the existence of overlapping cDNAs indicate that, in the bovine genome, the α3 and α5 genes overlap at their 3′ ends. This fact is probably due to inefficient transcription termination, as a result of weak polyadenylation signals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1997.68020488.x
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  • 2
    Electronic Resource
    Electronic Resource
    Twenty-nail dystrophy of childhood—two cases in one family (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental dermatology 9 (1984), S. 0 
    Details
    ISSN: 1365-2230
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Two siblings suffering from the so-called twenty-nail dystrophy are described. This syndrome is characterized by the appearance in early childhood of changes affecting all the nails: moderate koilonychia, marked accentuation of longtitudinal ridges and loss of lustre of the nail plates.These two cases, the first reported in the same family, confirm that the twenty-nail syndrome is a clinical entity which can be differentiated from other diseases involving all twenty nail plates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2230.1984.tb00866.x
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  • 3
    Electronic Resource
    Electronic Resource
    Optical anisotropy of Langmuir–Blodgett sapphyrin films (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 77 (2000), S. 3164-3166 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The polarization dependence of the optical reflectivity for sapphyrin layers deposited by the Langmuir–Blodgett technique onto a gold substrate has been measured. The experimental results show that characteristic reflectance anisotropy spectroscopy (RAS) spectra are related to layers of different thicknesses. In order to interpret the measured spectral features, the anisotropy of the optical properties of the sapphyrin molecule has been evaluated by using a semi-empirical quantum chemistry approach. The results show clearly that the main RAS structures are related to the electronic properties of the sapphyrin molecules. In particular, two different regimes are observed. Below one (true) monolayer, the optical signal remains very low and structureless, in agreement with the sapphyrin molecules being stacked with their planes almost perpendicular to the substrate plane. Above one monolayer, instead, a strong RAS signal related to the Soret band develops, suggesting that sapphyrin molecules lay more parallel to the substrate surface, in an ordered fashion. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1324983
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  • 4
    Electronic Resource
    Electronic Resource
    Italian rice varieties: historical data, molecular markers and pedigrees to reveal their genetic relationships (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two molecular marker approaches [amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR)] were employed to study genomic relationship among 96 rice cultivars. These included most of the best reputed Italian accessions. AFLP produced 461 fragments, 248 (53%) of which were polymorphic, SSR produced four to 11 alleles in the 12 genomic loci investigated. Genomic similarity was estimated independently for the two molecular marker techniques. Both AFLP and SSR dendrograms agree in splitting the cultivars into two main clusters: a small one, comprising four exotic accessions, and a larger one which could be split into four subgroups. These were also analysed on the basis of historical and pedigree information. This is the first report on the application of DNA polymorphism analysis to reveal genomic relationship among cultivated Italian rice germplasm. Results will be useful for breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00950.x
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  • 5
    Electronic Resource
    Electronic Resource
    Development of molecular markers to assess the level of introgression of Populus tremula into P. alba natural populations (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Morphological traits traditionally adopted to discriminate between Populus alba L. and P. tremula L. have frequently led to misclassifi-cation of their spontaneous hybrid P. × canescens Sm. Moreover, they may not be of any help in cases of spontaneous backcross phenomena. These limitations can be overcome by molecular markers, which are not environmentally influenced nor subjectively assessed. In this study, the effectiveness of amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers in species and hybrid discrimination was evaluated by analysing a set of reference samples of P. alba, P. tremula and P. × canescens. Species-specific and species-indicative AFLPs, as well as diagnostic SSR alleles, were recorded in both P. alba and P. tremula reference samples. The results allowed a clear distinction between the two poplar species and their hybrid. Using these diagnostic markers, a natural population of P. alba trees sampled along the Ticino river basin in northern Italy was analysed, and P. × canescens individuals, intermingled with P. alba trees, were detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00979.x
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  • 6
    Electronic Resource
    Electronic Resource
    Vine-1 retrotransposon-based sequence-specific amplified polymorphism for Vitis vinifera L. genotyping (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The sequence-specific amplification polymorphism (S-SAP) method, derived from the amplified fragment length polymorphism (AFLP) technique, produces amplified fragments containing retrotransposon long terminal repeat (LTR) sequence at one end and a host restriction site at the other. The development and application of this procedure to the LTR of the Vine-1 element from grapevine is reported. Two primers derived from one of the LTR sequences flanking the retrotransposon were used in combination with MseI degenerated primers on 15 grapevine accessions. S-SAP results were compared with AFLP data. The heterozygosity and gene diversity values were higher for S-SAP than for the AFLP procedure. Results show that S-SAP amplification is effective in identifying polymorphisms and defining genetic distances among cultivars, and could be used for fingerprinting and for ‘Traminer’ clone identification. To the contrary Vine-1 retrotransposon-based S-SAP was not able to distinguish ‘Pinot’ clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00965.x
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of genomic variability in transgenic sugarcane plants produced by Agrobacterium tumefaciens infection (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three transgenic sugarcane populations produced by Agrobacterium tumefaciens infection were analysed for the presence of genomic variability. Plants of the original cultivar, plants regenerated without transformation, as well as transformed and untransformed calli were used as control treatments. Amplified fragment length polymorphism (AFLP) of DNA extracted from leaves or calli assessed genomic profiling. The average DNA polymorphism within each population was determined by calculating the polymorphism index, while the extent of genomic dissimilarity among individual plants within transgenic populations was verified in unweighted pair group method using arithmetic averages dendrograms. The results showed that the production of transgenic sugarcane plants by A. tumefaciens infection is accompanied by limited but detectable genomic changes and that, on average, these occur at the same rate in plant populations carrying different transgenes. Main factors contributing to somaclonal variation in transgenic sugarcane plants have been verified by pre-existing DNA polymorphism into the donor genotype and in vitro culture steps during the transformation procedure. The relevant practical conclusion from this finding is that the AFLP analysis may be effectively used to identify individual transgenic plants with the least genomic deviation from the parental ones. The selected genotype would be conserved as cultivated sugarcane is asexually propagated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01021.x
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  • 8
    Electronic Resource
    Electronic Resource
    Exfoliative Dermatitis Following lntravesical Therapy with Mitomycin C (1985)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of dermatology 24 (1985), S. 0 
    Details
    ISSN: 1365-4632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-4362.1985.tb05824.x
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  • 9
    Electronic Resource
    Electronic Resource
    Exfoliative Dermatitis Following Intravesical Therapy with Mitomycin C (1985)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of dermatology 24 (1985), S. 0 
    Details
    ISSN: 1365-4632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-4362.1985.tb05532.x
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  • 10
    Electronic Resource
    Electronic Resource
    Cherry hemangioma: an SEM study (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 11 (1984), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study describes the examination of 10 examples of cherry hemangioma hy SEM. Morphologic features are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1984.tb00414.x
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