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Digitale Medien

Induction of FMN adenylyltransferase in the methanol utilizing yeast Candida boidinii (1977)

Eggeling, L. ; Sahm, H. ; Wagner, F.

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 1 (1977), S. 0

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ISSN: 1574-6968

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1574-6968.1977.tb00615.x

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Digitale Medien

Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimilating enzymes inHansenula polymorpha (1978)

Eggeling, L. ; Sahm, H.

Springer

Applied microbiology and biotechnology 5 (1978), S. 197-202

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ISSN: 1432-0614

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Summary The regulation of the synthesis of alcohol oxidase, catalase, formaldehyde dehydrogenase, and formate dehydrogenase was investigated in the methanol-utilizing yeastHansenula polymorpha during growth on different carbon and energy sources. When cells were grown on glucose, the enzymes of the dissimilatory methanol metabolism were not detected during the exponential phase of growth, but were formed in the late stationary phase without addition of methanol. Moreover, the enzymes were synthesized during growth on sorbitol, glycerol, ribose, and xylose. It was shown that the carbon catabolite insensitivity of the synthesis of methanol-specific enzymes is not limited to substrates that are slowly metabolized.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00579339

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Digitale Medien

Isolation and characterization of an obligate methanol-utilizing bacteriumMethylomonas M-15 (1975)

Sahm, H. ; Wagner, F.

Springer

Applied microbiology and biotechnology 1 (1975), S. 147-158

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ISSN: 1432-0614

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Summary A new obligate methylotrophic bacterium capable of rapid growth on methanol as its sole carbon and energy source was isolated. The organism grows only on methanol and not on methane or methylamine. It is a gram-negative, motile rod (0.5×1.5 μm) with a single polar flagellum and was therefore classified as a species ofMethylomonas. The doubling time was about 2 hr at pH 7.0, at a temperature of 30 to 36°C and at a methanol concentration of 1% (v/v). Cell suspensions were able to oxidize methanol, formaldehyde, and formate; therefore it seems that methanol-, formaldehyde-, and formate-oxidizing enzymes are present in the bacterium. While no hydroxy pyruvate reductase activity was found, the cell extract contained high hexulose phosphate-synthetase activity, indicating the assimilation of methanol via the ribulose phosphate-pathway. The protein content of the bacterium is 60% and the amino acid pattern indicates that this strain could serve as a good source of single cell protein.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00942211

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