Digitale Medien
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
44 (1985), S. 0
ISSN:
1471-4159
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Abstract: A procedure based on ion-exchange chromatography for chemical separation and radioimmunoassays for quantitation of substance P(SP), the SP(1–7), and C-terminal fragments, respectively, has been developed. The procedure allows the determination of these fragments in the presence of large (i.e., 50- to 100-fold) excess of parent compound. The chemical identity of isolated SP and fragments was studied with preparative electrophoresis on dilute agarose gel and with HPLC. The activity identified as SP(1–7) comigrated with the authentic standard whereas practically all activity isolated as C-terminal fragments comigrated with SP(5–11). The levels of C-terminal fragments in rat brain areas rich in SP and in spinal cord were 1–2% of those of parent compound. The levels of SP(1–7) were always higher, in the spinal cord markedly higher (three to five times). Postmortem storage of samples from brain and spinal cord indicated that SP(1–7) levels fell more rapidly than those of SP or C-terminal fragments.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1471-4159.1985.tb12874.x
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