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Digitale Medien

A helix-turn-strand structural motif common in α-β proteins (1990)

Rice, Phoebe A. ; Goldman, Adrian ; Steitz, Thomas A.

New York, NY : Wiley-Blackwell

Proteins: Structure, Function, and Genetics 8 (1990), S. 334-340

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ISSN: 0887-3585

Schlagwort(e): protein structure ; structural comparison ; α-β barrels ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: By exhaustive structural comparisons, we have found that about one-third of the α-helix-turn-β-strand polypeptides in α-β barrel domains share a common structural motif. The chief characteristics of this motif are that first, the geometry of the turn between the α-helix and the β-strand is somewhat constrained, and second, the β-strand contains a hydrophobic patch that fits into a hydrophobic pocket on the α-helix. The geometry of the turn does not seem to be a major determinant of the α-β unit, because the turns vary in length from four to six residues. However, the motif does not occur when there are few constraints on the geometry of the turn-for instance, when the turns between the α-helix and the β-strands are very long. It also occurs much less frequently in flat-sheet α-β proteins, where the topology is much less regular and the amount of twist on the sheet varies considerably more than in the barrel proteins. The motif may be one of the basic building blocks from which α-β barrels are constructed.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/prot.340080407

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Digitale Medien

Analysis of the interaction between human interleukin-5 and the soluble domain of its receptor using a surface plasmon resonance biosensor (1994)

Morton, Thomas A. ; Bennett, Donald B. ; Appelbaum, Edward R. ; [weitere]

Chicester [u.a.] : Wiley-Blackwell

Journal of Molecular Recognition 7 (1994), S. 47-55

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ISSN: 0952-3499

Schlagwort(e): Cytokine ; Receptor ; Biosensor ; Titration ; Calorimetry ; Association rate ; Dissociation rate ; Equilibrium analysis ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Medizin

Notizen: A surface plasmon resonance (SPR) biosensor was used to study the interaction of human interleukin-5 (hIL5) with its receptor. IL5 is a major growth factor in the production and activation of eosinophilis. The receptor for IL5 is composed of two subunits, α and β. The α subunit provides the specificity for IL5 and consist of an extracellular soluble domain, a single transmembrane region and a cytoplasmic tail. We expressed the soluble domain of the human IL5 receptor α subunit (shIL5Rα) and human IL5 (hIL5) in Drosophila. Both hIL5 and shIL5Rα were immobilized separately through amine groups onto the carboxylated dextran layer of sensor chips of the BIAcore™ (Pharmacia) SPR biosensor after N-hydroxysuccinimide/carbodiimide activation of the chip surface. Interactions were measured for the complementary macromolecule, either shIL5Rα or hIL5, in solution. Kinetics of binding of soluble analyst to immobilized ligand were measured and from this the association rate constant, dissociation rate constant and equilibrium dissociation constant (Kd) were derived. With immobilized shIL5Rα and soluble hIL5, the measured Kd was 2 nM. A similar value was obtained by titration calorimetry. The Kd for Drosophila expressed receptor and IL5 is higher than the values reported for proteins expressed in different systems, likely due to differences in the methods of interaction analysis used for differences in protein glycosylation. Receptor-IL5 binding was relatively pH independent between pH 6.5 and 9.5. Outside this range the dissociation rate increased with compressibility little increased in association rate. The values obtained for the interaction of hIL5 and shIL5Rα were found to depend on which component was immobilized; the Kd was 5.5 nM with immobilized hIL5 and soluble shIL5Rα. The SPR biosensor provides a unified methodology to measure the interaction properties of shIL5Rα and hIL5 derivatives, mutants and mimetic as well as to evaluate potential antagonists of the receptor-cytokine interaction.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jmr.300070107

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Digitale Medien

Pulsed-field electrophoresis in microlithographic arrays (1996)

Duke, Thomas A. J. ; Austin, Robert H. ; Cox, Edward C. ; [weitere]

Weinheim : Wiley-Blackwell

Electrophoresis 17 (1996), S. 1075-1079

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ISSN: 0173-0835

Schlagwort(e): Pulsed-field electrophoresis ; Microlithographic array ; Fractionation ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Transverse pulsed-field electrophoresis of DNA has been conducted in a silicon array engineered by optical lithography and the motion of individual molecules observed by fluorescence microscopy. In strong fields, the molecules can be maintained in highly stretched, linear conformations. When the field is switched through an obtuse angle, they head off in the new direction led by what was formerly their tail end. This backtracking gives rise to fractionation that is linear with molecular weight. A simple prescription exists for choosing the field parameters to obtain a particular range of separation. Since the molecular motions are much more uniform than those that occur in a gel, it is anticipated that the arrays will permit more efficient fractionation than traditional pulsed-field gel electrophoresis. Arrays suitably scaled down in size may be useful for pulsed-field sequencing.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150170616

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Digitale Medien

Multiplexed short tandem repeat polymorphisms of the Weber 8A set of markers using tailed primers and infrared fluorescence detection (1998)

Oetting, William S. ; Armstrong, Catherine M. ; Ronan, Shawn M. ; [weitere]

Weinheim : Wiley-Blackwell

Electrophoresis 19 (1998), S. 3079-3083

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ISSN: 0173-0835

Schlagwort(e): Multiplex polymerase chain reaction ; Tailed primers ; Gene mapping ; Short tandem repeat polymorphisms ; ODS ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Short tandem repeat polymorphism (STRP) markers have become important reagents for mapping genetic diseases. These markers are available as screening sets, which are located in all chromosomes at discrete intervals, allowing the entire genome to be analyzed. Mapping studies that include many individuals in the analysis necessitate the production of large numbers of genotypes. In an effort to increase the efficiency and lower the cost of using these STRP screening sets, we have divided the amplification primers of the Weber 8A screening set into groups that can be amplified in single polymerase chain reaction (PCR) amplification reactions, resulting in a reduction of both time and cost. Fluorescently-labeled amplification products were produced using a three primer reaction. The forward STRP amplification primer for each marker contained a 19 bp sequence at the 5′ end. A fluorescently-labeled primer, with a sequence identical to the 19 bp tail, was added to the amplification reaction as the sole source of fluorescent label. The STRP banding pattern is detected using an automated fluorescent DNA sequencer. Use of this multiplexed genomic screening set should greatly enhance the mapping of human disease loci.

Zusätzliches Material: 2 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/elps.1150191806

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Mass propagation of algae for photosynthetic gas exchange (1960)

Gaucher, Thomas A. ; Benoit, Richard J. ; Bialecki, Adolph

New York, NY [u.a.] : Wiley-Blackwell

Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 339-359

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ISSN: 0368-1467

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Engineering data on the design and operation of algal culture systems for photosynthetic gas exchange are virtually non-existent. The authors have conducted intermediate and definitive level engineering studies to characterize algal systems - with a view to extrapolating to larger-scale systems for life support in closed spaces.Light intensity, carbon dioxide concentration, and dilution rate were the principal parameters used to control the photosynthetic rate and, consequently, oxygen production. Carbon dioxide absorption rate, equilibrium density, and cellular growth rate were also investigated.It was found that: (1) properly jacketed high intensity, incandescent lamps provided a suitable light source for growing algae; and (2) physiologically safe (0·5 per cent) concentrations of carbon dioxide produced growth comparable to that obtained at higher concentrations.A dilution rate of nearly 0·1 volume change per hour produced the best oxygen yield (2·41 × 10-3 lb/h) for the definitive system. Maximum cell doubling time was 5·1 h. The highest culture density attained was 5·9 mm3/ml, and the maximum dry weight algae yield was 3·0 × 10-3 lb/h.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jbmte.390020309

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