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  • 1
    Electronic Resource
    Electronic Resource
    Quantitation of phenobarbital and its main metabolites in human urine (1975)
    Springer
    European journal of clinical pharmacology 9 (1975), S. 161-168 
    Details
    ISSN: 1432-1041
    Keywords: Conjugate-cleavage ; drug metabolism ; gas chromatography ; phenobarbital ; urinary excretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary A method for the quantitative determination of phenobarbital and free and conjugatedp-hydroxyphenobarbital in urine samples is described. The method includes initial extraction, purification on a small chromatographic column and finally determination by gas chromatography. The barbituric acids are methylated by trimethylanilinium hydroxide which serves as a “flash heater” methylating agent. The conjugate ofp-hydroxyphenobarbital, which appears to be a glucuronide, is hydrolysed with hydrochloric acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00614013
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