ZIB

1

Digitale Medien

HISTOCHEMISTRY OF MYELIN—XII ANIONIC STAINING OF MYELIN BASIC PROTEINS FOR HISTOLOGY, ELECTROPHORESIS AND ELECTRON MICROSCOPY (1971)

Adams, C. W. M. ; Bayliss, O. B. ; Hallpike, J. F. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 18 (1971), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract— Phosphotungstic acid haematoxylin, trypan blue and amidoblack techniques have been developed as anionic dye methods for staining myelin basic proteins. All methods displayed central and peripheral nervous system myelin in histochemical prepa rations and stained brain basic proteins in electrophoretic polyacrylamide gels: phosphotungstic acid haematoxylin appeared to be the most selective of these techniques. Electron photomicrographs of peripheral nerve stained by phosphotungstic acid haematoxylin showed that the major part of myelin basic protein is located in the period dense line. The basic proteins stained by phosphotungstic acid haematoxylin showed an early loss in rat sciatic nerve undergoing Wallerian degeneration and had completely disappeared from the centre of 20 plaques of multiple sclerosis.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb11966.x

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2

Digitale Medien

HISTOCHEMISTRY OF MYELIN. XIII DIGESTION OF BASIC PROTEIN OUTSIDE ACUTE PLAQUES OF MULTIPLE SCLEROSIS (1971)

Adams, C. W. M. ; Hallpike, J. F. ; Bayliss, O. B.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 18 (1971), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract— Plaques of multiple sclerosis from a patient with a short clinical history were investigated by qualitative and quantitative histochemical methods. One of three plaques examined showed perivenous lymphocytic infiltration; this plaque was regarded as a particularly early acute lesion. In this plaque a relatively wide zone of diminished staining for basic protein extended outwards around the edge of the lesion. A narrower and irregular zone of diminished cerebroside staining was also seen around the plaque. Staining for phosphoglycerides and cholesterol was relatively normal up to the edge of the lesion; no zone of reduced staining for these lipids was seen outside the plaque. Proteolytic activity was increased throughout the lesion (pH 3.5 〉 7.4). The two less acute plaques showed no obvious loss of basic protein and cerebroside outside the plaque. Addendum: Two further acute plaques of multiple sclerosis obtained recently, showed a similar loss of basic protein outside of lesion.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1971.tb00010.x

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3

Digitale Medien

HISTOCHEMISTRY OF MYELIN–XV. CHANGES IN THE MYELIN PROTEINS OF THE PERIPHERAL NERVE UNDERGOING WALLERIAN DEGENERATION–ELECTROPHORETIC AND MICRODENSITOMETRIC OBSERVATIONS (1972)

Adams, C. W. M. ; Csejtey, J. ; Hallpike, J. F. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 19 (1972), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: The chronological order of changes in rat peripheral nerve proteins during Wallerian degeneration has been investigated by microdensitometric and electrophoretic techniques. Both methods revealed an early loss of myelin proteins. The histochemical microdensitometric study showed a very substantial early loss of stainable protein basic groups and a somewhat slower progressive loss of the major protein component of peripheral nerve myelin (the J band). The electrophoretic study showed an early loss of both the J band protein and the slower-moving basic protein band. The histochemical study also suggested that some cerebroside may be lost in the early stage of Wallerian degeneration. It is concluded that degradation of myelin proteins is an initial event in the process of myelin breakdown.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1972.tb05114.x

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4

Digitale Medien

HISTOCHEMISTRY OF MYELIN XIV PERIPHERAL NERVE MYELIN PROTEINS: ELECTROPHORETIC AND HISTOCHEMICAL CORRELATIONS (1972)

Csejtey, J. ; Hallpike, J. F. ; Adams, C. W. M. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 19 (1972), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract— Myelin from the peripheral nervous system has been shown to contain two basic protein components and an electrophoretically slower-moving major protein, the ‘J’ band. The ‘J’ band protein cannot be selectively removed by aqueous or organic solvents and does not correspond to proteolipid or acidic protein. Histochemical stains applied to peripheral nervous systems myelin proteins separated by polyacrylamide electrophoresis indicate that ‘J’ band protein is analogous with the neurokeratin of the nerve sheath. Trypanophilia observed histochemically in unfixed myelin is principally due to basic proteins. With prolonged tryptic digestion ‘J’ band protein is degraded. Thus, previous classifications of myelin proteins based on trypsin sensitivity have been modified. All peripheral nervous system myelin proteins should be regarded as trypsin-sensitive, the basic protein being relatively more and the ‘J’ band protein relatively less susceptible.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1972.tb01481.x

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5

Digitale Medien

PROTEOLYTIC ACTIVITY AND BASIC PROTEIN LOSS IN AND AROUND MULTIPLE SCLEROSIS PLAQUES: COMBINED BIOCHEMICAL AND EIISTOCHEMICAL OBSERVATIONS (1972)

Einstein, E. R. ; Csejtey, J. ; Dalal, K. B. ; [weitere]

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 19 (1972), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Abstract— This combined histochemical and biochemical study has shown that acid proteinase activity (PH 3.5) is increased around histologically-defined active plaques of multiple sclerosis (MS). Biochemical estimation showed that the enzyme is more active in most samples of ‘normal’ white matter in MS than in controls. A gradient of enzyme activity was observed: control white matter-white matter distant from plaqueclose white matter-edgsplaque. Both electrophoretic and histochemical techniques revealed a reduction or absence of basic (encephalitogenic) protein in the plaques. Electrophoresis showed a diminution of encephalitogenic protein outside some plaques. Phospholipids that remain on the base-line of thin-layer chromatoplates were shown to be predominantly phosphoinositides combined with encephalitogenic protein

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1972.tb01382.x

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6

Digitale Medien

Biological Sciences: Cellular Lipid Inclusions in the White Matter in Multiple Sclerosis (1971)

BAYLISS, O. B. ; ADAMS, C. W. M.

[s.l.] : Nature Publishing Group

Nature 233 (1971), S. 264-265

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] Seventeen plaques from eight cases of multiple sclerosis and white matter from sixteen control patients, who had died from other neurological diseases, were fixed in routine saline-formol and transferred to 1% calcium acetate?10% formalin. Frozen sections were stained with haematoxylin-eosin, Sudan ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/233264a0

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7

Digitale Medien

Subcellular Preparation of Peripheral Nerve Myelin (1968)

ADAMS, C. W. M. ; ABDULLA, Y. H. ; TURNER, D. R. ; [weitere]

[s.l.] : Nature Publishing Group

Nature 220 (1968), S. 171-173

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ISSN: 1476-4687

Quelle: Nature Archives 1869 - 2009

Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik

Notizen: [Auszug] In discussion at a symposium at Liblice2, Professor E. Gutmann and Dr H. Koenig suggested that we should try to prepare a residue of myelin from dorsal spinal roots by plucking out their axons with forceps after treating these peripheral nerves with a glycine-diethylamine buffer3. This suggestion ...

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1038/220171a0

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8

Digitale Medien

Reappraisal of osmium tetroxide and OTAN histochemical reactions (1968)

Adams, C. W. M. ; Bayliss, O. B.

Springer

Histochemistry and cell biology 16 (1968), S. 162-166

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Osmium tetroxide and OTAN histochemical reactions have been reappraised following recent publications in this journal by Ellbder and Lojda. Provided that the standard OTAN reaction is used on frozen sections of conventional thickness, unsaturated hydrophilic polar lipids are stained in an orange or red shade while unsaturated hydrophobic non-polar lipids are stained brown-black or black. We have confirmed the anomaly — pointed out by Elleder and Lojda — that the “polar lipid” reaction of the atherosclerotic plaque is essentially extinguised by acetone. Solubility and blockading methods provide circumstantial evidence that Elleder and Lojda's assumed OsO4-protein reactions are due to the lipid moiety of a lipoprotein complex.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00280614

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9

Digitale Medien

Schiff reactions with lipids and the disputed terminal rinse with hydrochloric acid (1971)

Adams, C. W. M. ; Bayliss, O. B.

Springer

Histochemistry and cell biology 28 (1971), S. 220-224

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary We have been concerned with the effect of 3N HCl on the reaction-products of lipids and Schiff's reagent in the PAS and plasmal reactions. Our conclusions are: 1. We agree with Elleder and Lojda (1971) that the intensity of the modified PAS and plasmal reactions is reduced if a terminal 3N HCl rinse is used. However, we consider that the term “rinse” is not synonomous with a 5–10 min wash or bath. 2. We do not agree that this reduction is substantial. After a 1 min rinse with 3N HCl, it amounts to only 9.1% with the plasmal and 25.4% with the modified PAS reaction. 3. We do not agree that this modest reduction is necessarily all due to the release of fatty (or glycollic) aldehydes from conjugation with Schiff's reagent; it could equally well be due to suppression of phospholipid (or other anion) staining by the residual basic fuchsin in Schiff's reagent. 4. We do not agree that the plasmal (or PAS) reaction of brain lipids on paper is nearly extinguished by a terminal rinse in 3N HCl: such a result is possibly due to imperfect washing of chromatograms. With a terminal rinse of 1 min, the reactions were reduced by 31.5% and nil, respectively. 5. We do not agree that “the influence of an acid mounting medium” is of any practical significance with these histochemical reactions. 6. Finally we wish to reiterate that the staining of phospholipids (or other anions) by the residual basic fuchsin in Schiff's reagent is potentially a very real hazard in the application of the modified PAS and plasmal methods. Our experiments suggest that this hazard can be obviated by the use of the brief terminal 3N HCl rinse. Even if the acid does remove part of the reaction product with some Schiff methods, it is perhaps safer on these occasions to lose some of the baby with the bath-water.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00305676

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10

Digitale Medien

Osmium tetroxide as a histochemical and histological reagent (1967)

Adams, C. W. M. ; Abdulla, Y. H. ; Bayliss, O. B.

Springer

Histochemistry and cell biology 9 (1967), S. 68-77

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ISSN: 1432-119X

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary From the evidence discussed it can be concluded that osmium tetroxide (OsO4) would be reduced to black OsO2 (or an equivalent compound) by the ethylene bonds of liquid or solid cis-unsaturated lipids or by the Δ 5-double bond in cholesterol in solid state in tissues. No evidence has been obtained to suggest that OsO4 is either reduced or bound by proteins and polysaccharides in tissue-sections.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00281808

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