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Digitale Medien

DETERMINATION OF STAPHYLOCOCCAL ENTEROTOXINS A AND B IN VARIOUS FOOD EXTRACTS, USING STAPHYLOCOCCAL CELLS CONTAINING PROTEIN A (1980)

ARESON, P. D. W. ; CHARM, S. E. ; WONG, B. L.

Oxford, UK : Blackwell Publishing Ltd

Journal of food science 45 (1980), S. 0

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ISSN: 1750-3841

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A radioimmunoassay for Staphylococcus enterotoxin using Staphylococcus aureus cells containing protein A has already been developed. This technique has been employed in our laboratory to detect staphylococcal enterotoxins A and B in six different food materials. Food constituents that interfere with solid state radioimmunoassays do not affect this free solution method to the same extent. The protein A procedure may be used to generate a single representative standard curve, from which toxin levels in other foods may be quantitated. The sensitivity of this assay is 0.1 ng/ml for enterotoxin A and 0.5 ng/ml for enterotoxin B.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1365-2621.1980.tb02628.x

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Digitale Medien

A culture unit system for the study of responses of mycorrhizal and non-mycorrhizal seedling to treatments (1990)

Melhuish, J. H. ; Wong, B. L. ; McQuattie, C. J.

Springer

Plant and soil 129 (1990), S. 157-163

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ISSN: 1573-5036

Schlagwort(e): axenic conditions ; mycorrhizae ; Pinus taeda ; Pisolithus tinctorius

Quelle: Springer Online Journal Archives 1860-2000

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Abstract The culture unit described was designed to provide a method whereby the experimental condition of the substrate solution can be maintained or manipulated while retaining the axenic integrity of the system. The culture unit was equipped with a modified polypropylene lid allowing entry and exit of treatment solutions that had been previously sterilized. Loblolly pine (Pinus taeda L.) inoculated by Pisolithus tinctorius (Pers.) Coker and Couch was grown axenically for up to six months in this culture unit system. Growth of roots and mycorrhizal morphology in this system were similar to those observed in other systems.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00032408

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Digitale Medien

Enzyme inactivation with shearing (1970)

Charm, S. E. ; Wong, B. L.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 12 (1970), S. 1103-1109

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ISSN: 0006-3592

Schlagwort(e): Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Enzymes subjected to shearing in a viscometer are partially inactivated. It is possible with viscometry to calculate the degree of inactivation that occura when an enzyme solution flows through a capillary tube. When shear rate × exposure time is less than 104, there is little or no inactivation.The masa average shear-rate × time or shear, for laminar flow in a cylindrical tube is simply 16L/3D. It is surprising that for a single pass through a tube, the masa average shear is independent of flow rate and shear rate.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260120615

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