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  • 1
    Electronic Resource
    Electronic Resource
    Characteristics of lead adsorption byUndaria pinnatifida (1995)
    Springer
    Biotechnology letters 17 (1995), S. 345-350 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary We reportUndaria pinnatifida as a potential biosorbent for lead removal.U. pinnatifida exhibited approximately 350 mg of lead uptake capacity per gram of dry biomass within a pII range of 3 to 4. The major cellular component ofU. pinnatifida in lead binding was thought as alginic acid existing in the cell wall.U. pinnatifida adsorbed lead ion selectively over other alkaline metal ions such as K+, Na+, Mg++ and Ca++. It was confirmed by using instrumental analysis including EDX and XPS thatU. pinnatifida adsorbed lead ions on the surface of itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01190651
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of pH on the molecular weight of poly-3-hydroxybutyric acid produced by Alcaligenes sp. (1995)
    Springer
    Biotechnology letters 17 (1995), S. 389-394 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The molecular weight of a polymer is an important parameter characterizing the physical properties of the polymer. The effect of pH on the molecular weight of poly-3-hydroxybutyric acid (PHB) produced by Alcaligenes sp. K-912 were investigated. PHB having higher molecular weight was obtained when cultivated under high pH conditions compared to the results cultivated under low pH conditions. The polydispersity index was almost the same as 1.6. But the cell mass was decreased as the pH became higher. The molecular weight was mainly determined in the early cultivation period and molecular weight difference could be explained by calculating the number of polymer chains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130795
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Removal of phosphate in a sequencing batch reactor by Staphylococcus auricularis (2000)
    Springer
    Biotechnology letters 22 (2000), S. 1549-1552 
    Details
    ISSN: 1573-6776
    Keywords: anaerobic/aerobic switching ; phosphate ; sequencing batch reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A sequencing batch reactor (SBR) was used to remove phosphate in biological wastewater treatment as an alternative to the activated sludge process, in order to improve the low removal efficiency of phosphate and the operational instability. After a cycle of 2 h anaerobic and 4 h aerobic conditions, phosphate removal was optimized. The removal efficiencies of 5 and 50 mg phosphate l−1 by Staphylococcus auricularis under repeated anaerobic and aerobic conditions were above 90%. These results showed that a long adaptation time, one of the major problems in biological phosphate removal process, was overcome by SBR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005628816350
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Novel method using antibiotics for the determination of the rate-limiting step in the secretion pathway of glucoamylase from recombinant yeast (1996)
    Springer
    Biotechnology techniques 10 (1996), S. 257-262 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A novel method for the determination of the rate-limiting step in the protein secretion pathway in recombinant strains of Saccharomyces cerevisiae is proposed which used several antibiotics that specifically inhibit the processing enzymes in the secretion pathway. This novel method has advantages such as the relative simplicity of the experiments, non-requirement of secretion mutants, and absence of strain effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184025
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Development of a bioreactor for high density culture of hairy roots (1993)
    Springer
    Biotechnology techniques 7 (1993), S. 859-862 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of agitation and aeration on the growth of carrot hairy roots were investigated. When hydrodynamic stress index was above 0.001 cm/s, the growth rate of hairy roots decreased sharply. When volumetric O2 transfer coefficient was high, the specific growth rate was also high. However, the specific growth rate approached the maximum value when the volumetric O2 transfer coefficient was over 4 h−1 . It is therefore necessary to maintain low hydrodynamic stress and high volumetric oxygen transfer for high density culture of hairy roots. By considering hydrodynamic stress and oxygen transfer, a novel bioreactor type was suggested for hairy roots cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00156362
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Optimizations of carotenoid biosynthesis by controlling sucrose concentration (1990)
    Springer
    Biotechnology letters 12 (1990), S. 905-910 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of sucrose onDaucus carota cell size and biosynthesis of carotenoid were investigated. At high concentration of sucrose, the growth rate and the final cell mass were low due to the substrate inhibition, and cell size was small compared to the result at low sucrose concentration. However, very high level of specific carotenoid content was obtained from small sized cells compared to the condition from large sized cells. The biosynthesis of carotenoid can be increased by optimizing the cell culture method as follow: (i) cultivate the cell initially at low sucrose concentration to increase the cell growth rate, and (ii) when cell mass reaches a certain level, increase the sucrose concentration to a high level to make the cell size small and to increase the biosynthesis of carotenoid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022588
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Improved treatment of tannery wastewater using Zoogloea ramigera and its extracellular polymer in an activated sludge process (1996)
    Springer
    Biotechnology letters 18 (1996), S. 917-922 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary When Zoogloea ramigera and its extracellular polymer were daily added to the laboratory scale activated sludge reactor treating tannery wastewater, more than 15% of the COD concentration (COD; 151 mg/l) and 32% of the turbidity (NTU; 9.2) were removed compared with a controlled reactor (COD; 336 mg/l, NTU; 43.5) operated without any additions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154621
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Reactivation of Horseradish peroxidase in organic solvent using extraction (2000)
    Springer
    Biotechnology letters 22 (2000), S. 485-489 
    Details
    ISSN: 1573-6776
    Keywords: enzyme ; extraction ; Horseradish peroxidase ; organic solvent ; reactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005660131552
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  • 9
    Electronic Resource
    Electronic Resource
    Biosensor for the monitoring of hydrogen peroxide using poly(vinyl alcohol) membrane system (1991)
    Springer
    Biotechnology techniques 5 (1991), S. 453-458 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A biosensor system for continuous on-line monitoring of hydrogen peroxide concentration was developed employing catalase and a poly(vinyl alcohol)/poly(tetra fluoro ethylene) bilayer membrane system, Catalase was entrapped between poly(vinyl alcohol) membrane layer and poly(tetra fluoro ethylene) membrane layer outside of the galvanic type DO probe. Since poly(vinyl alcohol) membrane has non-porous, hydrophilic characteristics, the difference in hydrogen peroxide concentration between inside and outside of the membrane was therefore approximately 100 times. The developed hydrogen peroxide sensor has a wide linear range of hydrogen peroxide sensing more than 140 mM and favourable dynamic response characteristics. The sensor showed also good operational stability, rapid response time, and long life time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00155492
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of broth rheology with cell morphology inCephalosporium fermentation (1992)
    Springer
    Biotechnology techniques 6 (1992), S. 501-506 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Rheological properties inCephalosporium acremonium fermentation were quantitatively analysed. We obtained the correlation for consistency index (parameter of power law model) which could be estimated from the cell concentrations corresponding to morphological types. The correlated equation predicted the broth viscosity well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02447821
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    Paper (German National Licenses)
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