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  • 1
    Electronic Resource
    Electronic Resource
    Characteristics of lead adsorption byUndaria pinnatifida (1995)
    Springer
    Biotechnology letters 17 (1995), S. 345-350 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary We reportUndaria pinnatifida as a potential biosorbent for lead removal.U. pinnatifida exhibited approximately 350 mg of lead uptake capacity per gram of dry biomass within a pII range of 3 to 4. The major cellular component ofU. pinnatifida in lead binding was thought as alginic acid existing in the cell wall.U. pinnatifida adsorbed lead ion selectively over other alkaline metal ions such as K+, Na+, Mg++ and Ca++. It was confirmed by using instrumental analysis including EDX and XPS thatU. pinnatifida adsorbed lead ions on the surface of itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01190651
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  • 2
    Electronic Resource
    Electronic Resource
    Removal of copper using marine brown alga, Undaria Pinnatifida modified with oxime (1996)
    Springer
    Biotechnology techniques 10 (1996), S. 923-928 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary As oxime is selective for Cu2+, oxime groups were introduced to the cell wall of alga by glutaraldehyde. Such modified biomass showed high affinity for Cu2+, which resulted in the increase of copper sorption capacity about 4.5 times higher than that of natural alga. For pH range from 2.5 to 3.0, only Cu2+ were removed by alga biomass modified with oxime, while other heavy metal ions such as Ca2+,Cd2+,Pb2+ were not adsorbed. By changing pH, selective recovery of Cu2+ was achieved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180395
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Novel method using antibiotics for the determination of the rate-limiting step in the secretion pathway of glucoamylase from recombinant yeast (1996)
    Springer
    Biotechnology techniques 10 (1996), S. 257-262 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A novel method for the determination of the rate-limiting step in the protein secretion pathway in recombinant strains of Saccharomyces cerevisiae is proposed which used several antibiotics that specifically inhibit the processing enzymes in the secretion pathway. This novel method has advantages such as the relative simplicity of the experiments, non-requirement of secretion mutants, and absence of strain effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184025
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  • 4
    Electronic Resource
    Electronic Resource
    Biosensor for the monitoring of hydrogen peroxide using poly(vinyl alcohol) membrane system (1991)
    Springer
    Biotechnology techniques 5 (1991), S. 453-458 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A biosensor system for continuous on-line monitoring of hydrogen peroxide concentration was developed employing catalase and a poly(vinyl alcohol)/poly(tetra fluoro ethylene) bilayer membrane system, Catalase was entrapped between poly(vinyl alcohol) membrane layer and poly(tetra fluoro ethylene) membrane layer outside of the galvanic type DO probe. Since poly(vinyl alcohol) membrane has non-porous, hydrophilic characteristics, the difference in hydrogen peroxide concentration between inside and outside of the membrane was therefore approximately 100 times. The developed hydrogen peroxide sensor has a wide linear range of hydrogen peroxide sensing more than 140 mM and favourable dynamic response characteristics. The sensor showed also good operational stability, rapid response time, and long life time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00155492
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  • 5
    Electronic Resource
    Electronic Resource
    Reactivation of Horseradish peroxidase in organic solvent using extraction (2000)
    Springer
    Biotechnology letters 22 (2000), S. 485-489 
    Details
    ISSN: 1573-6776
    Keywords: enzyme ; extraction ; Horseradish peroxidase ; organic solvent ; reactivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005660131552
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  • 6
    Electronic Resource
    Electronic Resource
    Development of a bioreactor for high density culture of hairy roots (1993)
    Springer
    Biotechnology techniques 7 (1993), S. 859-862 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effects of agitation and aeration on the growth of carrot hairy roots were investigated. When hydrodynamic stress index was above 0.001 cm/s, the growth rate of hairy roots decreased sharply. When volumetric O2 transfer coefficient was high, the specific growth rate was also high. However, the specific growth rate approached the maximum value when the volumetric O2 transfer coefficient was over 4 h−1 . It is therefore necessary to maintain low hydrodynamic stress and high volumetric oxygen transfer for high density culture of hairy roots. By considering hydrodynamic stress and oxygen transfer, a novel bioreactor type was suggested for hairy roots cultivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00156362
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    New taxol assay method using tubulin-assembly stimulation (1995)
    Springer
    Biotechnology techniques 9 (1995), S. 885-890 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A novel taxol determination method which involves the tubulin-assembly stimulation is described. The tubulin-assembly was monitored by turbidity change at 350nm. In a limited range of taxol concentration (0 to 24 μM), taxol stimulated tubulin-assembly linearly. And this linear relation was observed from 20min to 30min after the reaction started. Bioactive derivatives of taxol, such as cephalomanin and 7-epi-10-deacetyltaxol also stimulated the tubulin-assembly. However, baccatin III, which was known as less active taxol derivative did not stimulate tubulin assembly. This result showed that the stimulation of tubulin assembly has a relationship with the antimiotic activity. This assay method have several advantages. 1) Time required for the measurement is relatively short. 2) Multiple samples can be measured simultaneously. 3) It can remove interference of less active taxane compounds more selectively than immuno-assay. Consequently, this method can be used to determine taxol concentration in biological samples. Especially, this method can be used for large scale selection of cell line and primary screening of new antimiotic compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00158541
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  • 8
    Electronic Resource
    Electronic Resource
    The effects of sodium bisulfite in extraction of PHB by hypochlorite (1995)
    Springer
    Biotechnology techniques 9 (1995), S. 709-712 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Molecular weight of PHB becomes low when using a hypochlorite extraction method for PHB separation. This disadvantage was overcome by adding sodium bisulfite which is an anti-oxidant. The molecular weight drop was decreased from 30 % to 14 % by adding sodium bisulfite. It was also found that raising the pH to 12 and the addition of a surfactant resulted in the improvement of the PHB purity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00159234
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of broth rheology with cell morphology inCephalosporium fermentation (1992)
    Springer
    Biotechnology techniques 6 (1992), S. 501-506 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Rheological properties inCephalosporium acremonium fermentation were quantitatively analysed. We obtained the correlation for consistency index (parameter of power law model) which could be estimated from the cell concentrations corresponding to morphological types. The correlated equation predicted the broth viscosity well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02447821
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  • 10
    Electronic Resource
    Electronic Resource
    Improved treatment of tannery wastewater using Zoogloea ramigera and its extracellular polymer in an activated sludge process (1996)
    Springer
    Biotechnology letters 18 (1996), S. 917-922 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary When Zoogloea ramigera and its extracellular polymer were daily added to the laboratory scale activated sludge reactor treating tannery wastewater, more than 15% of the COD concentration (COD; 151 mg/l) and 32% of the turbidity (NTU; 9.2) were removed compared with a controlled reactor (COD; 336 mg/l, NTU; 43.5) operated without any additions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154621
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    Paper (German National Licenses)
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