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  • 1
    Digitale Medien
    Digitale Medien
    Calcium-binding protein regucalcin increases calcium-independent proteolytic activity in rat liver cytosol (1992)
    Springer
    Molecular and cellular biochemistry 112 (1992), S. 89-95 
    Details
    ISSN: 1573-4919
    Schlagwort(e): Regucalcin ; calcium-binding protein ; proteinase ; rat liver cytosol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, isolated from rat liver cytosol, on neutral proteolytic activity in the hepatic cytosol was investigated. The Ca2+-requiring proteinase required 5–10 µM Ca2+ for maximal activity in the presence of a protein substrate (globin). The proteinase activity was markedly elevated by the addition of regucalcin (0.25–2.0 µM) in the absence or presence of Ca2+ (5.0 µM) added. The effect of regucalcin, however, was the greater in the absence of Ca2+ than that in the presence. The pronounced effect of regucalcin on the proteinase activity was also seen in the presence of 1.0 mM EGTA with or without Ca2+ (5.0 µM). In the absence of Ca2+, the regucalcin-increased proteinase activity was clearly inhibited by the presence of anti-regucalcin antiserum (diluted to 240-fold), leupeptin (20 and 200 µg/ml), and heavy metals (25 µM cadmium or 25 µM zinc), although the inhibition was not complete at the concentration used. The present findings suggest that regucalcin increases proteolytic activity in rat liver cytosol, and that regucalcin may activate Ca2+-independent neutral cysteinyl-proteinase.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229647
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  • 2
    Digitale Medien
    Digitale Medien
    Potential sensitivity of hepatic specific protein regucalcin as a marker of chronic liver injury (1997)
    Springer
    Molecular and cellular biochemistry 167 (1997), S. 187-190 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; liver injury ; hepatitis ; diagnostic marker
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract A novel calcium-binding protein regucalcin has been shown to be specifically expressed in the liver of various specifies including human. Regucalcin concentration in the serum of patients with chronic liver injury was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. Serum samples were obtained from 42 persons who were diagnosed as liver disorder. Serum regucalcin concentration in all patients was in the range of 3.7-69.6 ng/ml, although regucalcin was not entirely seen in the serum of normal subjects (10 persons) without hepatitis. Meanwhile, in 18 patients with liver injury, serum glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities were normal value (less than 40 I.U./l). Serum GOT and GPT activities from 24 patients showed a comparatively higher level (50-234 I.U./l). The present results demonstrate the potential sensitivity of regucalcin as a marker of chronic liver injury. (Mol Cell Biochem 167: 187-190, 1997)
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006859121897
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  • 3
    Digitale Medien
    Digitale Medien
    Inhibitory effect of calcium-binding protein regucalcin on ribonucleic acid synthesis in isolated rat liver nuclei (1997)
    Springer
    Molecular and cellular biochemistry 173 (1997), S. 169-175 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; calcium ; nuclear RNA synthesis ; regenerating ratliver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, a Ca2+-bindingf protein isolated from rat livercytosol, on ribonucleic acid (RNA) synthesis in the nuclei of normal ratliver and of regenerating rat liver was investigated. The liver weight at 1day after partial hepatectomy was increased about 50% of that ofsham-operated (control) rats. Calcium chloride (1.0-20 µM Ca2+ asfinal concentration) was added into the reaction mixture of nuclear RNAsynthesis. RNA synthesis was established by incorporation of [3H]-uridine5'-triphosphate (UTP) into the nuclear RNA. Addition of Ca2+ (5 and 10µM) caused a significant increase of RNA synthesis in the nuclei fromcontrol rat liver. Such effect of Ca2+ was potentiated in the nuclei ofregenerating liver; nuclear RNA synthesis was increased about 2 fold by the1.0 and 2.5 µM Ca2+ addition. The stimulatory effect of Ca2+ wassignificantly inhibited by the presence of a-amanitin (10-8 M), an inhibitorof RNA polymerase II. The presence of regucalcin (0.25 and 0.5 µM)significantly inhibited RNA synthesis in the nuclei from control rat liverand from regenerating rat liver. The inhibitory effect of regucalcin wasremarkable in the presence of EGTA (0.5 mM), and it was weakened by theaddition of Ca2+ (5 µM). Such regucalcin effect was not seen in thepresence of a-amanitin. The presence of anti-regucalcin IgG in the reactionmixture significantly increased RNA synthesis in the nuclei from control ratliver, indicating that the endogenous regucalcin may be involved in nuclearRNA synthesis. The present resuits demonstrate that regucalcin can inhibitnuclear RNA synthesis in rat liver. Regucalcin may have an inhibitory rolein the regulation of liver nuclear RNA synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006833111236
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  • 4
    Digitale Medien
    Digitale Medien
    Stimulatory effect of calcium administration on regucalcin mRNA expression is attenuated in the kidney cortex of rats ingested with saline (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 275-281 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; gene expression ; calmodulin ; spontaneous hypertensive rats ; rat kidney cortex
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats ingested with saline was investigated. The alteration in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb of open reading frame). Rats were freely given saline as drinking water for 7 days. Regucalcin mRNA levels in the kidney cortex were suppressed by saline ingestion. When calcium chloride (10 mg Ca/100 g body weight) was intraperitoneally administered to rats ingested with saline for 7 days, the effect of calcium administration to increase regucalcin mRNA levels was weakened by saline ingestion. Such effect was also seen by the administration of 2.5 and 5 mg Ca/100 g. Regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were not appreciably increased by the administration of calcium (10 mg/100 g). Meanwhile, calcium content in the kidney cortex was significantly elevated by the administration of calcium (10 mg/100 g) to normal rats. This increase was weakened in saline-ingested rats. Moreover, Ca2+/calmodulin-dependent protein kinase activity in the cytosol of kidney cortex was significantly decreased by saline ingestion. These results suggest the possibility that saline ingestion-induced suppression of regucalcin mRNA expression in the kidney cortex is partly involved in the attenuation of Ca2+ signalling.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006898910955
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  • 5
    Digitale Medien
    Digitale Medien
    Effect of calcium-binding protein regucalcin on Ca2+ transport system in rat liver nuclei: stimulation of Ca2+ release (1992)
    Springer
    Molecular and cellular biochemistry 113 (1992), S. 63-70 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; calcium transport ; rat liver nuclei
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on Ca2+ transport in rat liver nuclei was investigated. Ca2+ uptake and release were determined with a Ca2+ electrode. Ca2+ uptake increased dependent on adenosine triphosphate (ATP; 0.5-2.0 mM), while the uptake was negligible in the presence of 2 mM ADP or AMP. Regucalcin (0.5–2.0 μM) had no effect on Ca2+ uptake following addition of 2.0 mM ATP. Meanwhile, Ca2+, which accumulated in the nuclei during 10 min after ATP addition, was significantly released by the addition of regucalcin. This release was dose-dependent (0.1–2.0 μM). Vanadate (100 μM) and guanosine triphosphate (100 μM) did not cause a significant release of Ca2+ from the nuclei. Trifluoroperazine (TFP; 50 μM), an antagonist of calmodulin, significantly increased Ca2+ release from the nuclei. The presence of regucalcin (0.5 μM) further enhanced the TFP effect. These results indicate that regucalcin stimulates Ca2+ release from liver nuclei, and that the effect is not influenced by calmodulin antagonist. The finding suggests that regucalcin can regulate the Ca2+ transport system in rat liver nuclei.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00230886
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  • 6
    Digitale Medien
    Digitale Medien
    Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay (1993)
    Springer
    Molecular and cellular biochemistry 122 (1993), S. 65-68 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; tissue concentration ; liver ; kidney ; rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The concentration of calcium-binding protein regucalcin in the tissues of rats was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. In male rats (5 weeks old), regucalcin was most pronounced in the liver. Liver regulcalcin concentration was about 0.1μM, when it was calculated with regucalcin molecular weight of 28,800. The relatively higher level of regucalcin was also found in the kidney as compared with that of the skeletal muscle, duodenum, testis, lung, heart, spleen, cerebral cortex and hippocampus. Similarly in female rats, regulacalcin was remarkable in the liver, and appeared only slightly in the kidney. Thus, the tissue distribution of regucalcin in rats was specific in the liver. The concentration of regucalcin in the liver was altered with increasing age of rats; liver regucalcin level linearly increased during 5 weeks old after birth of male rats, and then began to decrease gradually. The results coincided with the previous observation of Northern blot analyses by using liver regucalcin cDNA as a probe. The present finding clearly demonstrates that regucalcin is specifically synthesized in the liver of rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00925738
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  • 7
    Digitale Medien
    Digitale Medien
    Hepatic calcium-binding protein regucalcin is released into the serum of rats administered orally carbon tetrachloride (1994)
    Springer
    Molecular and cellular biochemistry 131 (1994), S. 173-179 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; liver injury ; carbon tetrachloride
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The change in calcium-binding protein regucalcin, mainly localized in liver, in the liver and serum of rats received a single oral administration of carbon tetrachloride (50%; 1.0 ml/100 g body weight) was investigated. The change of regucalcin mRNA levels in the liver was analyzed by Northern blotting using liver regucalcin cDNA (0.6 kb). At 10 and 24 h after the administration, liver regucalcin mRNA levels were reduced markedly. Moreover, regucalcin concentration in the liver and serum was estimated by enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG. Administration of carbon tetrachloride (CCl4) induced a significant decrease in liver regucalcin concentration and a corresponding elevation of serum regucalcin concentration at 24 h after the administration. An appreciable increase in serum regucalcin concentration was seen at 2 h after the administration. Meanwhile, serum transaminases (GOT and GPT) activities were significantly increased by CCl4 administration, indicating that liver injury is induced. The present study demonstrates that hepatic regucalcin is released into the serum of rats administered orally CCl4, suggesting that the estimation of serum regucalcin is a useful tool for diagnosis of liver injury.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00925954
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  • 8
    Digitale Medien
    Digitale Medien
    Expression of hepatic calcium-binding protein regucalcin mRNA is decreased by phenobarbital administration in rats (1994)
    Springer
    Molecular and cellular biochemistry 141 (1994), S. 15-19 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; gene expression ; phenobarbital ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of phenobarbital on the expression of calcium-binding protein regucalcin mRNA in rat liver was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.9 kb of open reading frame). Phenobarbital (4, 8 and 12 mg/ 100 g body weight) was intraperitoneally administered to rats 3 times with 24 h intervals, and the animals were sacrificed by bleeding at 24 h after the last administration. The hepatic regucalcin mRNA levels were markedly reduced by phenobarbital administration. This decrease was about 50% of control level with the 12 mg/100 g dose. Moreover, the hepatic regucalcin concentration was significantly decreased by the administration of phenobarbital (12 mg/100 g), although the serum regucalcin concentration was not altered appreciably. Meanwhile, serum transaminases (GOT and GPT) activities were not increased by the administration of phenobarbital (4 and 12 mg/100 g). The present study demonstrates that the expression of hepatic regucalcin mRNA is decreased by phenobarbital administration in rats, suggesting that regucalcin does not have a role in drug metabolism related to phenobarbital.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00935586
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  • 9
    Digitale Medien
    Digitale Medien
    Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: Involvement of glucose, insulin and calcium as stimulating factors (1995)
    Springer
    Molecular and cellular biochemistry 142 (1995), S. 35-41 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; insulin ; calcium ; gene expression ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of refeeding on the expression of Ca2+-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level (about 150–170% of fasted rats). Liver regucalcin concentration was appreciably increased by refeeding, although it was not altered by fasting. The oral administration of glucose (2 g/kg body weight) to fasted rats caused a significant increase in hepatic regucalcin mRNA level. Moreover, hepatic regucalcin mRNA level was clearly elevated by a single subcutaneous administration of insulin (10 and 100 U/kg) to fasted rats. The hormonal effect was not further enhanced by the simultaneous administration of calcium chloride (250 mg Ca/kg) to fasted rats, although calcium administration stimulated regucalcin mRNA expression in the liver. The present study suggests that the expression of hepatic regucalcin mRNA stimulated by refeeding is significantly involved in the action of insulin and/or calcium as stimulating factors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00928911
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  • 10
    Digitale Medien
    Digitale Medien
    Calcium administration increases calcium-binding protein regucalcin concentration in the liver of rats (1995)
    Springer
    Molecular and cellular biochemistry 143 (1995), S. 53-58 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; protein synthesis ; metabolic degradation ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The alteration of regucalcin concentrations in the liver and serum of rats administered orally calcium is investigated. Rats received a single oral administration of calcium chloride solution (25, 50 and 75 mg Ca/100 g body weight). The administration of calcium (50 mg/100 g) produced a significant increase in liver regucalcin concentration between 30 and 180 min after the administration, while serum regucalcin concentration was not altered appreciably. The effect of calcium administration increasing liver regucalcin concentration was also seen with the dose of 25 mg/100 g. When liver cytosol prepared from normal rats was incubated for 6 h in the presence of 10 μM Ca2+, the cytosolic regucalcin concentration at 3 and 6 h of incubation was decreased about 20% (p〈0.05) as compared with the value at zero time point, indicating that the presence of Ca2+ does not inhibit the decomposition of liver cytosolic regucalcin. Moreover, serum regucalcin concentration was not significantly altered by the incubation for 6 h at 37°C, indicating a stability of regucalcin in rat serum. This suggests that the calcium administration-induced in liver regucalcin concentration is not based on the inhibition of regucalcin release from liver to serum. The present study demonstrates that regucalcin in the liver is clearly increased by calcium administration, presumably due to stimulating the protein synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00925926
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