ISSN:
1573-8272
Schlagwort(e):
expanded bed adsorption
;
α-lactalbumin
;
milk proteins
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Abstract The technique of expanded bed adsorption is originally designed for a direct recovery of proteins from fermentor feedstocks. In this article we describe the use of expanded bed adsorption for the recovery of α-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. α-Lactalbumins are Ca2+- binding proteins. Upon Ca2+ removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of α-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1023/A:1008098009623
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