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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Acta neuropathologica 26 (1973), S. 93-106 
    ISSN: 1432-0533
    Schlagwort(e): Leucodystrophy, Metachromatic, Juvenile ; Sulfatide Accumulation ; Biochemistry ; Myelination ; Metabolic Derangement
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Clinical history, histopathological, ultrastructural and biochemical observations are presented in a juvenile MLD with onset at 8 years and death at 20 years. The findings are compared with related ones in different age groups, and current views on sulfatide metabolism and enzymatic pathogenesis in MLD are briefly entertained. It is postulated that the relatively well established morphological and chemical stability of the nervous tissue at the time of appearance of the metabolic derangement was an important factor in the pathomechanism of the disease process in this particular variant.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1432-0878
    Schlagwort(e): Neurosecretory tract (eel) ; Transport of hormones ; Arginine-vasotocin ; Ichthyotocin ; Biochemistry ; light microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Une étude pharmacologique et histologique des transformations enregistrées le long du tractus neurosécrétoire est réalisée chez l'anguille européenne après hypophysectomie totale. Les animaux, sacrifiés entre 8 jours et 150 jours après hypophysectomie, sont d'abord examinés sur le plan quantitatif en suivant le devenir de l'arginine-vasotocine et de l'istotocine dans les parties antérieure (noyau préoptique) et caudale du tractus neurosécrétoire. L'étude est ensuite effectuée sur un plan qualitatif, en suivant, pour les mêmes territoires et les mêmes périodes de temps, les transformations notées pour le neurosécrétat. Les résultats obtenus au moyen de ces deux techniques d'investigation sont comparés. 1. Sur le plan quantitatif, l'expérience montre que l'évolution de l'arginine-vasotocine est différente de celle de l'isotocine, et qu'une distinction peut être faite entre les parties antérieure et caudale du tractus neurosécrétoire. En règle générale, et particuhèrement pour le premier peptide, lorsqu'il y a chute de concentration au niveau préoptique il y a augmentation de concentration dans la partie caudale, ce qui permet de traduire le cheminement des hormones. 2. L'expérience montre également que les hormones sont toujours présentes, mais subissent des chutes de concentration très importantes. 3. Le contrôle histologique confirme les résultats précédents, et montre des variations importantes dans la quantité et la colorabilité du neurosécrétat.
    Notizen: Summary A pharmacological and histological study of the transformations of the neurosecretory material observed along the neurosecretory tract of the hypophysectomized eel has been performed. The animals, sacrificed within a period of 8 to 150 days after hypophysectomy, have been first examined on a quantitative level by registrating the concentration of arginine-vasotocin and ichthyotocin in the anterior (preoptic nucleus) and caudal parts of the neurosecretory tract. The study was then transferred to a qualitative level for the same regions and post-operative times, to follow up the transformations of the neurosecretory material. The results obtained with these two approaches of investigation are compared. 1. On the quantitative level, the experiments showed that the arginine-vasotocin behaves differently if compared with ichthyotocin, and that a difference exists between the anterior and caudal parts of the neurosecretory tract. In general, a decrease of concentration in the preoptic nucleus is followed by an increase of concentration in the caudal part. This is particularly obvious in the case of the arginine-vasotocin. This result speaks in favour of the transport of hormones along the neurosecretory tract. 2. The experiment showed also that the hormones are always present, but show an important decrease in concentration. 3. The histological control confirmed the pharmacological results, showing great variations of the quantity and stainability of the neurosecretory material.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1432-0878
    Schlagwort(e): Intestine, Lampetra fluviatilis ; Serotonin-, dopamine-, noradrenaline-containing neurons ; Identification by histochemistry ; Electron microscopy ; Biochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Monoamine-containing neurons in the gut of Lampetra fluviatilis are characterized by histochemical, electron microscopical and biochemical methods. Strongly yellow fluorescent, probably serotonin-containing intrinsic neurons are found along the entire length of the intestine. Their processes aggregate to form large bundles of mainly non-terminal axons, constituting a subepithelial fibre plexus. This subepithelial, ganglion cell comprising plexus is connected to a wide-meshed subserosal plexus which has ganglion cells of different size and few varicose, single axons. Intermingled with both plexus there occur — in the anterior and middle but not in the preanal portion of the lamprey intestine — scattered green fluorescent intrinsic perikarya, emanating faintly green fluorescent, poorly varicosed axons. The formaldehyde-induced neuronal fluorophores conform to serotonin (yellow fluorescent compound), noradrenaline, and dopamine (green fluorescent substance), as revealed in microspectrofluorimetric recordings. The electron microscopical analysis of the yellow fluorescent intrinsic neurons in the terminal hindgut shows nerve cell pericarya and axons equipped with a typical population of occasional small granular and many large granular vesicles (750–1600 Å). The number and opacity of cores of the small and the osmiophilia of the cores of the large granular vesicles are significantly increased following short-term treatment with 5,6-dihydroxytryptamine. Long-term treatment with 5,6- or 5,7-dihydroxytryptamine provokes severe signs of ultrastructure impairment and eventual degeneration in the supposed serotonin-containing axons, besides indications of piling-up of organelles in the non-terminal axons due to arrest of axonal transport. Chromatography of acid extracts from the lamprey intestine, gills and kidney reveals the presence of serotonin (besides another unidentified indoleamine) and dopamine and noradrenaline in the gut, but only dopamine in the brain. The detection of serotonin, noradrenaline and dopamine in the lamprey gut is confirmed by chemical determinations. The occurrence of intrinsic serotonin-, noradrenaline- and dopamine-containing neurons in the gut of Lampetra fluviatilis deviates from the established pattern of innervation of the vertebrate intestine and is considered to be a remnant of an autonomic innervation principle common in invertebrates.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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