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  • 2010-2014
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  • 1990-1994  (10)
  • 1955-1959
  • 1910-1914
  • 1890-1899
  • 1990  (10)
  • Agrobacterium
  • 1
    Electronic Resource
    Electronic Resource
    Glyphosate degradation byAgrobacterium radiobacter isolated from activated sludge (1990)
    Springer
    Journal of industrial microbiology and biotechnology 6 (1990), S. 219-221 
    Details
    ISSN: 1476-5535
    Keywords: Agrobacterium ; Achromobacter ; Glyphosate degradation ; Biodegradation ; Sequencing batch reactor ; Activated sludge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Two species of bacteria capable of growth onN-phosphonomethylglycine (glyphosate) were isolated from a bench scale sequencing batch reactor degrading a waste stream containing glyphosate. The enrichment and isolation medium contained defined salts and glyphosate as the sole carbon and energy source. Glyphosate was stoichiometrically degraded to aminomethylphosphonic acid (AMPA). The bacteria have been identified asAgrobacterium radiobacter andAchromobacter Group V D.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01577700
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  • 2
    Electronic Resource
    Electronic Resource
    3-Fluorobenzoate enriched bacterial strain FLB 300 degrades benzoate and all three isomeric monofluorobenzoates (1990)
    Springer
    Archives of microbiology 153 (1990), S. 193-199 
    Details
    ISSN: 1432-072X
    Keywords: 3-Fluorobenzoate ; 4-Fluorocatechol ; Orthopathway ; Chemotaxonomy ; rRNA analysis ; Quinone pattern analysis ; Polyamine pattern analysis ; Agrobacterium ; Rhizobium branch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bacterial strain FLB300 was enriched with 3-fluorobenzoate as sole carbon source. Besides benzoate all isomeric monofluorobenzoates were utilized. Regioselective 1,2-dioxygenation rather than 1,6-dioxygenation yielded 4-fluorocatechol and minimized the production of toxic 3-fluorocatechol. Degradation of 4-fluorocatechol was mediated by reactions of ortho cleavage pathway activities. Chemotaxonomic and r-RNA data excluded strain FLB300 from a phylogenetically defined genus Pseudomonas and suggested its allocation to the alpha-2 subclass of Proteobacteria in a new genus of the Agrobacterium-Rhizobium branch.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247820
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  • 3
    Electronic Resource
    Electronic Resource
    T-DNA presence and opine production in tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281 (1990)
    Springer
    Plant molecular biology 14 (1990), S. 111-117 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium ; conifers ; DNA hybridization ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018552
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  • 4
    Electronic Resource
    Electronic Resource
    Improved binary vectors for Agrobacterium-mediated plant transformation (1990)
    Springer
    Plant molecular biology 14 (1990), S. 269-276 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium ; binary vectors ; ColE1 replicon ; lac Z′ ; pRi replicon ; plant transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Improved plant transformation vectors were constructed which utilize the pRiHRI origin of replication for highly stable maintenance in Agrobacterium tumefaciens, the ColE1 origin of replication for high copy maintenance in Escherichia coli, and a gentamycin resistance gene as a strong selectable marker for bacteria. Concise T-DNA elements were engineered with border sequences from the TL-DNA of pTiA6, the Tn5 neomycin phosphotransferase gene (npt II) expressed from either CaMV 35S or mannopine synthase (mas) promoters, and the lac Z′ gene segment from pUC18 as a source of unique restriction sites as well as an insertional inactivation marker for cloned DNA. The order of T-DNA components in all vectors is left border, plant marker cassette, lac Z′, and right border, respectively. The prototype vector, pCGN1547, was shown to be very stable in A. tumefaciens strain LBA4404 and to act as an efficient donor of T-DNA in tomato transformation experiments. Use of the other vectors is also described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018567
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  • 5
    Electronic Resource
    Electronic Resource
    The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction (1990)
    Springer
    Plant molecular biology 15 (1990), S. 747-753 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium ; hairy roots ; T-DNA ; geotropism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The T-DNA regions of three strains of Ri plasmids 1855, 8196, 2659 (agropine, mannopine and cucumopine type respectively) share two highly conserved regions flanking a non-homologous central part [1,2]. We have cloned segments of the cucumopine Ri plasmid 2659 T-DNA in the binary vector system Bin 19 and infected carrot discs with recombinant Agrobacterium strains. We show here that the central non-conserved region is crucial in hairy root induction as it is sufficient to induce rooting on the apical (auxin-rich) surface of carrot discs; in order to observe rooting on the basal (auxin-depleted) side of the discs, a longer T-DNA fragment, also encompassing part of the right conserved region, had to be utilized in conjunction with a Agrobacterium strain carrying aux genes. Differences of growth properties in culture are exhibited by roots transformed with different fragments of pRi 2659 T-DNA, although all transformed roots show the plagiotropic behaviour typical of hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016124
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  • 6
    Electronic Resource
    Electronic Resource
    Pea convicilin: structure and primary sequence of the protein and expression of a gene in the seeds of transgenic tobacco (1990)
    Springer
    Planta 180 (1990), S. 461-470 
    Details
    ISSN: 1432-2048
    Keywords: Agrobacterium ; Convicilin ; Nicotiana (transgenic) ; Pisum (convicilin gene) ; Transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Convicilin, a trimeric globulin of pea (Pisum sativum L.) seeds, is closely related to vicilin and composed of polypeptides of 68.2 kilodaltons. A partial copy DNA (cDNA) clone encoding convicilin was isolated, sequenced, and used to select a convicilin gene from a pea genomic library. A part of the genomic clone was sequenced to obtain the coding sequences missing in the cDNA clone and a further 1 kilobase 5′ to the start of transcription were also obtained. The entire sequence of convicilin was deduced from the combined genomic and cDNA sequences. The complete gene encoding convicilin was transferred to tobacco (Nicotiana tabacum L.) and the characteristics of its expression in the seeds of transgenic plants were studied. An unprocessed polypeptide, which was found only in the seeds of the transgenic plants, was identical in size to pea convicilin, and was recognized by vicilin antibodies. Convicilin, which does not undergo posttranslational cleavage in peas, was partially processed to polypeptides of a relative molecular mass (Mr) of approx. 50000 in transgenic tobacco seeds. There was a twofold variation in the level of convicilin accumulated by the mature seeds of a number of transgenic plants and this was well correlated with the number of gene copies incorporated in the different transformants. In the seeds of tobacco plants that contained a single copy of the transferred gene it was estimated that convicilin comprised up to 2% of the seed protein. Thus, using a combination of gene sequencing and expression in a heterologous host we believe we have characterized the gene corresponding to theCvc locus, whereas the gene described by D. Bown et al. (1988, Biochem J.,251, 717–726) probably encodes a minor convicilin-related protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411442
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  • 7
    Electronic Resource
    Electronic Resource
    The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open reading frames (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 256-266 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Vir proteins ; virB DNA sequence ; Membrane proteins ; T-DNA transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium tumefaciens genetically transforms plant cells by transferring a copy of its T-DNA to the plant where it is integrated and stably maintained. In the presence of wounded plant cells this process is activated and mediated by the products of the vir genes which are grouped into six distinct loci. The largest is the virB locus spanning 9.5 kb. Transposon mutagenesis studies have shown that virB gene products are required for virulence but their functions remain largely unknown. To provide information relevant to understanding the function of VirB polypeptides, the nucleotide sequence of the virB operon from a nopaline plasmid, pTiC58, is presented here. Eleven open reading frames (ORFs) are predicted from this sequence. The predicted sizes of 10 of the 11 VirB polypeptides are verified by specific expression in Escherichia coli. Only the product of the smallest ORF potentially encoding a 5.8 kDa polypeptide has not been detected. The initiation of translation of five virB ORFs occurs at codons that overlap the termination codons of the ORF immediately upstream; thus, translational coupling may be an important mechanism for efficient translation of the large virB polycistronic mRNA. Based on hydropathy plot analysis nine of the virB ORFs encode proteins that may interact with membranes; these data support the earlier hypothesis (Engstromm et al. 1987) that virB gene products may form a membrane pore or channel to mediate exit of the T-DNA copy (T-strands) from Agrobacterium into the plant cell. A comparison of the two published octopine virB sequences with the nopaline sequence presented here is made.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261729
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  • 8
    Electronic Resource
    Electronic Resource
    Agrobacterium tumefaciens T-DNA gene 6b stimulates rol-induced root formation, permits growth at high auxin concentrations and increases root size (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 1-10 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; 6b gene ; Oncogenes ; Tumour induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary All Agrobacterium tumefaciens strains studied up to now transfer an active 6b gene to plant cells. However, the role of this gene in natural tumour induction is unknown. Various effects of 6b on plant cell growth have been described, but the precise mechanism by which 6b causes these effects has not been elucidated. Earlier experiments indicated that the 6b gene might increase auxin sensitivity as do the A. rhizogenes rol genes. The 6b gene from Tm4 (T-6b) was therefore compared with the rolB and rolABC genes. Although T-6b was unable to induce root formation, it strongly interfered with root induction and root elongation. In rolABC/T-6b coinfection experiments on carrots, T-6b-transformed cells stimulated root outgrowth of rolABC-transformed cells, indicating that the biologically active T-6b product is diffusible. Carrot rolABC roots containing the T6b gene rapidly developed into unorganized calli. Nicotiana rustica roots with rolABC and T-6b continued their development, but became very large. Fragments of such roots formed callus at α-naphthaleneacetic acid concentrations which inhibited growth of rolABC and normal root fragments, suggesting that the role of 6b genes in natural tumour induction may be to reduce the inhibitory effects of high auxin levels and to keep cells in an undifferentiated state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315790
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  • 9
    Electronic Resource
    Electronic Resource
    Agrobacterium plasmids encode structurally and functionally different loci for catabolism of agrocinopine-type opines (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 465-473 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Opine catabolism ; Ti plasmids ; Agrocinopine ; Agrocin 84
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium tumefaciens strains C58, T37, K827 and J73, A. rhizogenes strains A4 and 15834, and A. radiobacter strain K299 were all susceptible to agrocin 84 and this sensitivity was enhanced in each case by addition of agrocinopines A and B. Analysis of transconjugants showed that sensitivity of strain A4 to agrocin 84 was encoded by pArA4a and not by the rhizogenic plasmid, pRiA4. The acc region of the A. tumefaciens nopaline-type Ti plasmid pTiC58, contained on the recombinant plasmid pTHH206, hybridized strongly to restriction fragments of plasmids from strains T37, K827, J73 and K299. Hybridizing fragment patterns generated with BamHI and EcoRI were identical among the four Ti plasmids while pAtK299 showed restriction fragment length polymorphisms at acc with the two enzymes. At moderate stringency, the pTiC58 acc region hybridized weakly to a single restriction fragment from the Ar plasmid of A. rhizogenes strain A4, but not to pTiBo542, which encodes catabolism of the closely related opines agrocinopines C and D. Plasmid pAtK84b of A. radiobacter strain K84 is induced for conjugal transfer by agrocinopines A and B. However, no hybridization was detected between this plasmid and acc from pTiC58 under conditions of moderate stringency. Like pTiC58, pAtK84b conferred transport of agrocinopines A and B on its host bacteria despite the absence of detectable sequence homology with the pTiC58-derived acc probe. However, unlike pTiC58, pAtK84b failed to confer sensitivity to or uptake of agrocin 84 on its bacterial host. These results indicate that at least four distinguishable systems exist for catabolism of the two agrocinopine opine families with the prototype locus, exemplified by acc from pTiC58, being strongly conserved among nopaline-type Ti plasmids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264455
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  • 10
    Electronic Resource
    Electronic Resource
    Oxygen-dependent desulphation of monomethyl sulphate by Agrobacterium sp. M3C (1990)
    Springer
    Biodegradation 1 (1990), S. 229-241 
    Details
    ISSN: 1572-9729
    Keywords: Agrobacterium ; desulphation ; monomethyl sulphate ; oxygenase ; methylotrophy ; sulphate ester degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agrobacterium sp. M3C, previously isolated from canal-water for its ability to grow on monomethyl sulphate, degraded this ester with stoichiometric liberation of inorganic sulphate. In contrast with the biodegradation of monomethyl sulphate in Hyphomicrobium sp., and of other longer-chain alkyl sulphates in Pseudomonas spp., the pathway in Agrobacterium appeared not to involve a sulphatase enzyme capable of catalysing ester-bond hydrolysis. No such sulphatase was detectable under a range of conditions of bacterial culture, or using various methods for preparing cell-extracts, or different assay conditions. There was no incorporation of 18O-label from H2 18O into the liberated inorganic sulphate. No methanol was detectable during biodegradation, and the organism was incapable of growth on methanol, and did not produce methanol dehydrogenase activity when grown on monomethyl sulphate. Tracer studies using mono[14C]-methyl sulphate indicated that formate serine and glycine were produced during the biodegradation. The presence of these amino acids, together with high activity of hydroxypyruvate reductase, indicated the operation of the serine pathway common in methylotrophs. Use of an oxygen electrode in conjunction with monomethyl[35S]sulphate showed that release of 35SO4 2- was dependent on availability of O2, and that there was equimolar stoichiometry among monomethyl sulphate degraded, O2 consumed and 35SO4 2- released. A proposed pathway for the degradation involved an initial mono-oxygenation to methanediol monosulphate with subsequent elimination of SO4 2- and concomitant formation of formaldehyde. The pathway was compared with degradation mechanisms for other C1 compounds and for other sulphate esters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00119760
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