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  • 2020-2022
  • 1990-1994  (6)
  • 1955-1959
  • 1920-1924
  • 1991  (6)
  • Industrial Chemistry
  • somaclonal variation
  • 1
    Electronic Resource
    Electronic Resource
    An evaluation of a somaclone ofDioscorea floribunda Mart & Gall (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 284-288 
    Details
    ISSN: 1420-9071
    Keywords: D. floribunda ; in vitro regeneration ; somaclonal variation ; diosgenin ; mixoploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 150 plants ofD. floribunda representing a single clone were regenerated from a stem tissue culture and regenerants were subjected to cytological, phenotypic and biochemical analysis from the pre-transfer stage to three vegetative growth cycles in the field. The plants could be subdivided into three cytological categories, namely, diploid, mosaic and tetraploid. Diploids, mosaics and the one tetraploid showed diversity amongst themselves with respect to internode length, content of chlorophyll and diosgenin. No marked difference in the length and nature of the leaf or in the type of stoma was recorded. Possible causes of the observed variation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01958162
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in phenotype and proteins in plants regenerated from cell suspensions of potato cv. BP1 (1991)
    Springer
    Euphytica 54 (1991), S. 41-44 
    Details
    ISSN: 1573-5060
    Keywords: Potato ; protein-DNA-binding ; SDS PAGE ; Solanum tuberosum ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Phenotypic variation, SDS-PAGE and protein-DNA binding were used to determine variation during the in vitro phase of potato plantlets derived from callus and cell suspensions. Of the 27 plantlets assessed. 3 displayed a low or abnormal growth, 16 normal growth which correlated well with the original explant and 9 showed strong or vigorous growth. Differences were not observed in the polypeptide profiles of these plantlets. However distinct differences in the protein-DNA-binding profiles occurred which correlated well with the phenotypic variation observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00145629
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Field resistance to late blight (Phytophthora infestans (Mont.) De Bary) in potato (Solanum tuberosum L.) somaclones associated with instability and pleiotropic effects (1991)
    Springer
    Euphytica 56 (1991), S. 75-80 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; Phytophthora infestans ; late blight ; adventitious regeneration ; somaclonal variation ; tissue culture ; mutation ; maturity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Adventitious regenerants (‘somaclones’) of ‘Bintje’ and their vegetative progeny were screened for field resistance to Phytophthora infestans as follows: the area under the disease progress curve was computed and correlated with resistance rating in ‘Bintje’ and reference varieties. The resistance rating of the somaclones was determined from this relationship. Clones with stable improved field resistance in successive years' trials were detected, however, most of such clones were also maturation mutants. Variation in resistance rating in clone replicates and between years was detected in most clones. The possible basis of the field resistance and reasons for its instability are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041746
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review (1991)
    Springer
    Euphytica 56 (1991), S. 269-285 
    Details
    ISSN: 1573-5060
    Keywords: disease resistance ; in vitro selection ; somaclonal variation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Somaclonal variation, i.e. the variation induced by cell and tissue culture, offers an opportunity to broaden the genetic variation of crops. As a result of somaclonal variation a wide range of plant characteristics can be altered. However, the selection of agronomically important traits, e.g. disease resistance, has many limitations. The efficiency of selection can be increased by the application of in vitro selection procedures. Selection strategies that may be applied to obtain disease resistant somaclonal variants are described. Their merits and limitations, in relation to the efficiency of the procedures, the frequency of disease resistant variants and the genetics of the resistance obtained, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042373
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Lotus corniculatus L.: Morphological and cytological analysis of regenerants from three sources of tissue and selected progeny (1991)
    Springer
    Plant cell, tissue and organ culture 25 (1991), S. 27-33 
    Details
    ISSN: 1573-5044
    Keywords: cytology ; leaf explants ; Lotus corniculatus ; protoplasts ; regeneration ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regenerants from three types of tissue, leaf explants (132 plants), leaf protoplasts (68 plants) and cotyledonary protoplasts (119 plants) of L. corniculatus cv Leo differed both morphologically and cytologically from control plants grown from seed. Four categories of chromosome number were found. The frequency and type of variation found in the chromosome numbers of regenerants reflected the method of plant regeneration. Regenerants with both normal and abnormal numbers of chromosomes produced progeny which were cytologically normal and showed only minor morphological changes when compared with control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033909
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. I. Double-layer culture technique (1991)
    Springer
    Euphytica 57 (1991), S. 251-257 
    Details
    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; double-layer technique ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calluses of spring and winter wheats (Triticum aestivum L.) were selected for Fusarium resistance in vitro, using the double-layer culture technique. Potato-dextrose agar medium in vials was inoculated with mycelia of Fusarium graminearum and F. culmorum. After one week, fungal cells were killed by autoclaving and the agar medium containing the thermostable toxic metabolites was overlayered with MS callus-growing medium. Later, wheat calluses were placed on the upper medium for 4–5 weeks, and from the surviving calluses plants were regenerated. R2 seedling populations from self-fertilized R1 plants of 4 varieties were tested for Fusarium resistance by artificial infections in the greenhouse, and 3% of the regenerated R2 plants have been found to be more resistant than the original cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039671
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    Paper (German National Licenses)
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