ISSN:
1432-072X
Schlagwort(e):
Gene bank
;
Plasmids pRK290
;
pRK2013
;
Rhodopseudomonas capsulata
;
Reconstitution
;
Phototroph negative mutants
;
Absorption spectra
;
Light harvesting complexes
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A gene bank of the phototrophic bacterium Rhodopseudomonas capsulata was constructed using the binary plasmid system pRK290/pRK2013. Fragments of about 20 kb of chromosomal DNA of R. capsulata strain 37b4 were inserted into the cloning vector pRK290. The hybrid plasmids of the gene bank, maintained in Escherichia coli HB101 were transferred by conjugation to R. capsulata strains defective in the photosynthetic apparatus with frequencies of 5×10-4 to 5×10-2. Phototrophically growing transconjugants occurred with frequencies of 5×10-7 to 5×10-6. Recombination between the hybrid plasmids and the R. capsulata chromosome was shown. The hybrid plasmid pRCF1002, carrying a 25 kb insert of R. capsulata wild type DNA, was isolated from one E. coli clone of the gene bank. It reconstituted some bacteriochlorophyll- and photosynthetic negative mutants to phototrophic growth.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00408373
