ISSN:
0884-3996
Keywords:
Alpha-fetoprotein
;
chemiluminescence
;
enzyme immunoassay
;
b̃-D-galactosidase
;
5-bromo-4-chloro-3-indolyl-b̃-D-galactopyranoside
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
We developed a sensitive chemiluminescent sandwich-type enzyme immunoassay (CLEIA) of alpha-fetoprotein (AFP) using b̃-D-galactosidase (b̃-gal) as a label and 5-bromo-4-chloro-3-indolyl-b̃-D-galactopyranoside as a substrate. The CL-EIA for AFP was performed using two monoclonal antibodies, one antibody is labelled with b̃-gal, the other is coated onto the inside surface of a polystyrene tube. The detection limit for AFP was 0.5 ng/mL, equivalent to 10 pg/assay tube. The coefficient of variation for within and between assay imprecision were 2.0%-4.9% (n = 10) and 4.4%-9.8% (n = 5), respectively. AFP values in serum determined by this method correlated well with those obtained by radioimmunoassay (n = 26, r = 0.99). This sensitive AFP assay can be performed within 4 h and can be used as a routine assay in clinical diagnosis.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bio.1170080302
