ISSN:
0749-503X
Keywords:
Saccharomyces cerevisiae
;
glycolysis
;
oscillation
;
carbon dioxide
;
mass spectrometry
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The rate of formation of carbon dioxide in cytoplasmic yeast extracts in an open system with continuous infusion of glucose was measured by membrane inlet mass spectrometry during glycolytic oscillations. The rate of CO2 production rose in the first third of each cycle to a maximum of about 100 μmol per ml yeast extract per hour and subsequently diminished to a final level of about 50 μmol per h. Measurements of the NADH light absorption under the same conditions revealed oscillations of relaxations type. The phase of high CO2 production could be related to the phase of the high NADH level, giving evidence that the flux in glycolysis is increased during the phase of high NADH concentration. Only half of the amount of injected glucose was metabolized to CO2 during the sustained oscillation, although free glucose did not accumulate.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320060310
