ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Cell structured support material (CSM) prepared from Wolffia arrhiza fronds were loaded with Saccharomyces cereevisiae by the propagation of yeast cells introduced in the aerenchyma. The loading process could be strongly accelerated by preventing the growth of freely suspended yeasts in the fermenter. The pathway of the inoculation through the stomata could be visualized by transparent light microscopy of amylase and protease treated CSM. Slices of the loaded CSM clearly showed that the framework of the inner chlorenchyma cells remains intact during the propagation of the included yeast cells. Most of the yeast cells were found to be localized as a dense suspension within unbroken cells. During long-term treatment of the loaded CSM at high convection, about one third of the yeasts was released into water with a constant rate greater than 0.34 h-1. The residual amount of yeast cells remained in a stable compartment from which further loss did not occur for the period tested (20-48 h). It is assumed that the stable compartment is identical with the interior of chlorenchyma cells. The results are discussed in relationship to the possible use of the immobilized biomass in fermentation.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370150203
